Dense, fast-moving regions of ionization called polar cap patches are known to occur in thehigh-latitudeFregion ionosphere. Patches are widely believed to be caused by convection of dense, sunlitplasma into a dark and therefore low-density polar cap ionosphere. This leads to the belief that patches are awinter phenomenon. Surprisingly, a long-term analysis of 3 years of ionospheric measurements from theSwarm satellites shows that large density enhancements occur far more frequently in local summer than localwinter in the Southern Hemisphere (SH). The reverse is true in the Northern Hemisphere (NH). Previouslyreported patch detections in the SH are reexamined. Detection algorithms using only a relative doubling testcount very small densityfluctuations in SH winter due to extremely low ambient densities found there,while much larger enhancements occurring in SH summer are missed due to especially high ambientdensities. The same problem does not afflict results in the NH, where ambient densities are more stableyear-round due to the ionospheric annual asymmetry. Given this new analysis, the definition of a patch as adoubling of the ambient density is not suitable for the SH. We propose a test for patches linked to long-termaveraged solarflux activity, characterized by the 81 day centered meanF10.7index. Importantly, thecurrent patch formation theory is at least incomplete in that it does not predict the observed lack of patchesin SH winter, or the many large enhancements seen in SH summer
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Approximate Bayesian computation methods for daily spatiotemporal precipitation occurrence simulation: ABC FOR PRECIP OCCURRENCE SIMULATION
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The application of animal manures to cropland is an important nutrient recycling strategy in many parts of the world. Commonly, aggregated manure wastes contain chemical stressors including veterinary antimicrobials, heavy metals, and antimicrobial resistance genes (ARGs) that can stimulate the development and proliferation of antimicrobial resistance (AMR). While the presence of antimicrobials in manure is well-documented, the co-occurrence of other potentially impactful chemical stressors in swine manure remains underreported. This study quantifies and analyzes correlations between antimicrobials, metals, and certain ARGs present in manure samples from swine farms in Iowa, United States. Relationships between chemical stressors and different stages of swine production or feed composition are also investigated. Results revealed substantial levels of tetracyclines [up to 1,260 µg g −1 dry weight (d.w.) of manure for oxytetracycline] detected in all samples. Tiamulin, two ionophores (monensin and lasalocid), and one macrolide (tilmicosin) were detected at maximum class concentrations of 9.4, 0.547, and 0.472 µg g −1 d.w., respectively. The median relative abundances of ermB and tetM were 0.13 and 0.17 copies g −1 wet weight (w.w.) manure (normalized to 16S gene), respectively. Additionally, high levels of copper (Cu), iron (Fe), and zinc (Zn) were detected in all samples, with maximum concentrations of 887, 1,900, and 2,100 µg g −1 d.w., respectively. Notably, uranium (U) was detected in 11 samples, at concentrations up to 0.77 µg g −1 . A global analysis of AMR-stressor relationships using Spearman’s rank correlation indicates Cu, and Ba are the most positively and significantly correlated with cytotoxic anhydrotetracycline (ATC) and/or anhydrochlortetracycline (ACTC) concentrations in all tested facilities (Cu-ATC: ρ = 0.67, p = 0.0093; Cu-ACTC: ρ = 0.75, p = 0.0022; Ba-ATC: ρ = 0.84, p = 0.0002). Interestingly, ermB and tetM genes were strongly, positively correlated to each other ( ρ = 0.92, p < 0.0001), suggesting possible co-selection, despite the absence of correlation between ARGs and tetracycline concentrations. This study demonstrates the complexity of interactions between antimicrobials, metals, and ARGs in multiple manure storage pits prior to cropland application.more » « less
