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Plant sap-feeding insects (Hemiptera) rely on bacterial symbionts for nutrition absent in their diets. These bacteria experience extreme genome reduction and require genetic resources from their hosts, particularly for basic cellular processes other than nutrition synthesis. The host-derived mechanisms that complete these processes have remained poorly understood. It is also unclear how hosts meet the distinct needs of multiple bacterial partners with differentially degraded genomes. To address these questions, we investigated the cell-specific gene-expression patterns in the symbiotic organs of the aster leafhopper (ALF),
Macrosteles quadrilineatus (Cicadellidae). ALF harbors two intracellular symbionts that have two of the smallest known bacterial genomes:Nasuia (112 kb) andSulcia (190 kb). Symbionts are segregated into distinct host cell types (bacteriocytes) and vary widely in their basic cellular capabilities. ALF differentially expresses thousands of genes between the bacteriocyte types to meet the functional needs of each symbiont, including the provisioning of metabolites and support of cellular processes. For example, the host highly expresses genes in the bacteriocytes that likely complement gene losses in nucleic acid synthesis, DNA repair mechanisms, transcription, and translation. Such genes are required to function in the bacterial cytosol. Many host genes comprising these support mechanisms are derived from the evolution of novel functional traits via horizontally transferred genes, reassigned mitochondrial support genes, and gene duplications with bacteriocyte-specific expression. Comparison across other hemipteran lineages reveals that hosts generally support the incomplete symbiont cellular processes, but the origins of these support mechanisms are generally specific to the host–symbiont system. -
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Whereas the gill chambers of jawless vertebrates open directly into the environment, jawed vertebrates evolved skeletal appendages that drive oxygenated water unidirectionally over the gills. A major anatomical difference between the two jawed vertebrate lineages is the presence of a single large gill cover in bony fishes versus separate covers for each gill chamber in cartilaginous fishes. Here, we find that these divergent patterns correlate with the pharyngeal arch expression of Pou3f3 orthologs. We identify a deeply conserved Pou3f3 arch enhancer present in humans through sharks but undetectable in jawless fish. Minor differences between the bony and cartilaginous fish enhancers account for their restricted versus pan-arch expression patterns. In zebrafish, mutation of Pou3f3 or the conserved enhancer disrupts gill cover formation, whereas ectopic pan-arch Pou3f3b expression generates ectopic skeletal elements resembling the multimeric covers of cartilaginous fishes. Emergence of this Pou3f3 arch enhancer >430 Mya and subsequent modifications may thus have contributed to the acquisition and diversification of gill covers and respiratory strategies during gnathostome evolution.
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null (Ed.)This study presents a new approach for detection and mapping of ancient slag heaps using 16-band multispectral satellite imagery. Understanding the distribution of slag (a byproduct of metal production) is of great importance for understanding how metallurgy shaped long-term economic and political change across the ancient Near East. This study presents results of slag mapping in Oman using WorldView-3 (WV3) satellite imagery. A semi-automated target detection routine using a mixed tuned matched filtering (MTMF) algorithm with scene-derived spectral signatures was applied to 16-band WV3 imagery. Associated field mapping at two copper production sites indicates that WorldView-3 satellite data can differentiate slag and background materials with a relatively high (>90%) overall accuracy. Although this method shows promise for future initiatives to discover and map slag deposits, difficulties in dark object spectral differentiation and underestimation of total slag coverage substantially limit its use. Resulting lower estimations of combined user’s (61%) and producer’s (45%) accuracies contextualize these limitations for slag specific classification. Accordingly, we describe potential approaches to address these challenges in future studies. As sites of ancient metallurgy in Oman are often located in areas of modern exploration and mining, detection and mapping of ancient slag heaps via satellite imagery can be helpful for discovery and monitoring of vulnerable cultural heritage sites.more » « less
