CRISPR‐Cas9 technology has been successfully applied in
In many organisms of biotechnological importance precise genome editing is limited by inherently low homologous recombination (HR) efficiencies. A number of strategies exist to increase the effectiveness of this native DNA repair pathway; however, most strategies rely on permanently disabling competing repair pathways, thus reducing an organism's capacity to repair naturally occurring double strand breaks. Here, we describe a CRISPR interference (CRISPRi) system for gene repression in the oleochemical‐producing yeast
- NSF-PAR ID:
- 10040276
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Biotechnology and Bioengineering
- Volume:
- 114
- Issue:
- 12
- ISSN:
- 0006-3592
- Format(s):
- Medium: X Size: p. 2896-2906
- Size(s):
- ["p. 2896-2906"]
- Sponsoring Org:
- National Science Foundation
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Summary The ability to edit plant genomes through gene targeting (
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