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1. N-(Trimethylsilyl)-2-amino-5-nitrothiazole: An Efficient Reagent for the Direct Synthesis of 2-Amino-5-nitrothiazole-Based Antimicrobial Agents

   https://doi.org/10.1055/s-0042-1752343  

   Koenig, Heidi N. ; Demeritte, Amethyst R. ; Livinghouse, Tom ; Nelson, Genevieve P. ( March 2022 , Synlett)

   Here we report the synthesis of a novel reagent designed to prepare 2-amino-5-nitrothiazole (ANT) amides and analogues in high yields. N-(Trimethylsilyl)-2-amino-5-nitrothiazole (N-(TMS)-ANT) was prepared in 99% yield via silylation of ANT using 1,1,1,3,3,3-hexamethyldisilazane (HMDS), trimethylsilyl chloride (TMSCl), and catalytic saccharin. N-(TMS)-ANT is a superb reagent for the preparation of ANT amides in excellent yields. Notably, cyclic anhydrides and base-sensitive acyl chlorides can be utilized with N-(TMS)-ANT to furnish ANT amides that are difficult to prepare by previously reported procedures.

    

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2. Amino mapping: possibility to visualize amino-N compounds in the rhizosphere of Zea Mays L.

   https://doi.org/10.1007/s00374-023-01754-0  

   Khosrozadeh, Sajedeh ; Guber, Andrey ; Nourbakhsh, Farshid ; Khalili, Banafshe ; Blagodatskaya, Evgenia ( July 2023 , Biology and Fertility of Soils)

   Abstract Understanding N uptake by plants, the N cycle, and their relationship to soil heterogeneity has generated a great deal of interest in the distribution of amino-N compounds in soil. Visualization of the spatial distribution of amino-N in soil can provide insights into the role of labile N in plant-microbial mechanisms of N acquisition and plant N uptake, but until now, it has remained technically challenging. Here, we describe a novel technique to visualize the amino-N distribution at the root-soil interface. The technique is based on time-lapse amino mapping (TLAM) using membranes saturated with the fluorogenic OPAME reagent ( O -phthalaldehyde and β-mercaptoethanol). OPAME in the membrane reacts with organic compounds containing a NH 2 functional group at the membrane-soil interface, generating a fluorescent product visible under UV light and detectable by a digital camera. The TLAM amino-mapping technique was applied to visualize and quantify the concentration of amino-N compounds in the rhizosphere of maize ( Zea Mays L.). A ten times greater amino-N concentration was detected in the rhizosphere compared to non-rhizosphere soil. The high content of amino-N was mainly associated with the root tips and was 3 times larger than the average amino-N content at seminal roots. The amino-N rhizosphere was 2 times broader around the root tips than around other parts of the roots. We concluded that TLAM is a promising approach for monitoring the fate of labile N in soils. However, the technique needs to be standardized for different soil types, plant species, and climate conditions to allow wider application. 

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3. Quantitative Chiroptical Sensing of Free Amino Acids, Biothiols, Amines, and Amino Alcohols with an Aryl Fluoride Probe

   https://doi.org/10.1021/jacs.9b07588  

   Thanzeel, F. Yushra ; Sripada, Archita ; Wolf, Christian ( September 2019 , Journal of the American Chemical Society)
4. Chiroptical sensing of amino acids, amines, amino alcohols, alcohols and terpenes with π-extended acyclic cucurbiturils

   https://doi.org/10.1039/D1OB00345C  

   Hassan, Diandra S. ; De los Santos, Zeus A. ; Brady, Kimberly G. ; Murkli, Steven ; Isaacs, Lyle ; Wolf, Christian ( May 2021 , Organic & Biomolecular Chemistry)

   null (Ed.)

   The efficiency and scope of two acyclic π-wall extended cucurbiturils, M2 and M3 , exhibiting rapidly interconverting helical conformers for chiroptical sensing of amines, amino acids, alcohols, and terpenes at micromolar concentrations in water is evaluated. The formation of 1 : 1 host–guest complexes results in spontaneous induction of circular dichroism signals that can be used for accurate determination of the absolute configuration and enantiomeric composition of the analyte based on a simple mix-and-measure protocol. 

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5. One-Pot Preparation of Enantiopure Fluorinated β-Amino Acid Precursors: Enantiopure Fluorinated β-Amino Acid Precursors

   https://doi.org/10.1002/ejoc.201402369  

   Jones, Joshua H. ; Appayee, Chandrakumar ; Brenner-Moyer, Stacey E. ( August 2014 , European Journal of Organic Chemistry)