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1. Worm-globule transition of amphiphilic pH-responsive heterografted bottlebrushes at air–water interface

   https://doi.org/10.1039/D3SM01635H  

   Kelly, Michael T; Zhao, Bin (February 2024, Soft Matter)

   Heterografted molecular bottlebrushes (MBBs) with side chains composed of poly(n-butyl acrylate) (PnBA) and pH-responsive poly(2-(N,N-diethylamino)ethyl methacrylate) (PDEAEMA, pKa = 7.4) have been shown to be efficient, robust, and responsive emulsifiers. However, it remains unknown how they respond to external stimuli at interfaces. In this work, the shape-changing behavior of six hetero- and homografted MBBs at air–water interfaces in response to pH changes and lateral compression was investigated using a Langmuir–Blodgett trough and atomic force microscopy. At a surface pressure of 0.5 mN m−1, PDEAEMA-containing MBBs showed no worm-globule transitions when the pH was increased from 4.0 to 10.0, at which PDEAEMA becomes insoluble in water. Upon lateral compression at pH 4.0, MBBs with a mole fraction of PDEAEMA side chains (xPDEAEMA) < 0.50 underwent pronounced worm-globule shape transitions; there was an increasing tendency for bottlebrushes to become connected with increasing xPDEAEMA. At xPDEAEMA = 0.76, the molecules remained wormlike even at high compression. These observations were presumably caused by the increased electrostatic repulsion between protonated PDEAEMA side chains in the subphase with increasing xPDEAEMA, hindering the shape change. At pH 10.0, MBBs with xPDEAEMA < 0.50 showed a lower tendency to change their wormlike morphologies upon compression than at pH 4.0. No shape transition was observed when xPDEAEMA > 0.50, attributed to the relatively high affinity toward water and the rigidity of PDEAEMA. This study revealed the shape-changing behavior of amphiphilic pH-responsive MBBs at air–water interfaces, which could be useful for future design of multicomponent MBBs for potential applications. 

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2. Local volume concentration, packing domains, and scaling properties of chromatin

   https://doi.org/10.7554/eLife.97604  

   Carignano, Marcelo A; Kroeger, Martin; Almassalha, Luay M; Agrawal, Vasundhara; Li, Wing Shun; Pujadas-Liwag, Emily M; Nap, Rikkert J; Backman, Vadim; Szleifer, Igal (September 2024, eLife)

   We propose the Self Returning Excluded Volume (SR-EV) model for the structure of chromatin based on stochastic rules and physical interactions. The SR-EVrules of returngenerate conformationally defined domains observed by single-cell imaging techniques. From nucleosome to chromosome scales, the model captures the overall chromatin organization as a corrugated system, with dense and dilute regions alternating in a manner that resembles the mixing of two disordered bi-continuous phases. This particular organizational topology is a consequence of the multiplicity of interactions and processes occurring in the nuclei, and mimicked by the proposed return rules. Single configuration properties and ensemble averages show a robust agreement between theoretical and experimental results including chromatin volume concentration, contact probability, packing domain identification and size characterization, and packing scaling behavior. Model and experimental results suggest that there is an inherent chromatin organization regardless of the cell character and resistant to an external forcing such as RAD21 degradation. 

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3. Evolution of Force Chains Explains the Onset of Strain Stiffening in Fiber Networks

   https://doi.org/10.1115/1.4055586  

   Sarkar, Mainak; Notbohm, Jacob (November 2022, Journal of Applied Mechanics)

   Abstract Fiber networks are the primary structural components of many biological structures, including the cell cytoskeleton and the extracellular matrix. These materials exhibit global nonlinearities, such as stiffening in extension and shear, during which the fibers bend and align with the direction of applied loading. Precise details of deformations at the scale of the fibers during strain stiffening are still lacking, however, as prior work has studied fiber alignment primarily from a qualitative perspective, which leaves incomplete the understanding of how the local microstructural evolution leads to the global mechanical behavior. To fill this gap, we studied how axial forces are transmitted inside the fiber network along paths called force chains, which continuously evolve during the course of deformation. We performed numerical simulations on two-dimensional networks of random fibers under uniaxial extension and shear, modeling the fibers using beam elements in finite element software. To quantify the force chains, we identified all chains of connected fibers for which the axial force was larger than a preset threshold and computed the total length of all such chains. To study the evolution of force chains during loading, we computed the derivative of the total length of all force chains with respect to the applied engineering strain. Results showed that the highest rate of evolution of force chains coincided with the global critical strain for strain stiffening of the fiber network. Therefore, force chains are an important factor connecting understanding of the local kinematics and force transmission to the macroscale stiffness of the fiber network. 

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4. Ordered domains in sheared dense suspensions: The link to viscosity and the disruptive effect of friction

   https://doi.org/10.1122/8.0000453  

   Goyal, Abhay; Del Gado, Emanuela; Jones, Scott Z.; Martys, Nicos S. (September 2022, Journal of Rheology)

   Monodisperse suspensions of Brownian colloidal spheres crystallize at high densities, and ordering under shear has been observed at densities below the crystallization threshold. We perform large-scale simulations of a model suspension containing over [Formula: see text] particles to quantitatively study the ordering under shear and to investigate its link to the rheological properties of the suspension. We find that at high rates, for [Formula: see text], the shear flow induces an ordering transition that significantly decreases the measured viscosity. This ordering is analyzed in terms of the development of layering and planar order, and we determine that particles are packed into hexagonal crystal layers (with numerous defects) that slide past each other. By computing local [Formula: see text] and [Formula: see text] order parameters, we determine that the defects correspond to chains of particles in a squarelike lattice. We compute the individual particle contributions to the stress tensor and discover that the largest contributors to the shear stress are primarily located in these lower density, defect regions. The defect structure enables the formation of compressed chains of particles to resist the shear, but these chains are transient and short-lived. The inclusion of a contact friction force allows the stress-bearing structures to grow into a system-spanning network, thereby disrupting the order and drastically increasing the suspension viscosity. 

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5. Search for long-lived charginos based on a disappearing-track signature using 136 fb$$^{-1}$$ of pp collisions at $$\sqrt{s}$$ = 13 TeV with the ATLAS detector

   https://doi.org/10.1140/epjc/s10052-022-10489-5  

   Aad, G.; Abbott, B.; Abbott, D. C.; Abed Abud, A.; Abeling, K.; Abhayasinghe, D. K.; Abidi, S. H.; Abramowicz, H.; Abreu, H.; Abulaiti, Y.; et al (July 2022, The European Physical Journal C)

   Abstract A search for long-lived charginos produced either directly or in the cascade decay of heavy prompt gluino states is presented. The search is based on proton–proton collision data collected at a centre-of-mass energy of $$\sqrt{s}$$ s  = 13 T $$\text {eV}$$ eV between 2015 and 2018 with the ATLAS detector at the LHC, corresponding to an integrated luminosity of 136 fb $$^{-1}$$ - 1 . Long-lived charginos are characterised by a distinct signature of a short and then disappearing track, and are reconstructed using at least four measurements in the ATLAS pixel detector, with no subsequent measurements in the silicon-microstrip tracking volume nor any associated energy deposits in the calorimeter. The final state is complemented by a large missing transverse-momentum requirement for triggering purposes and at least one high-transverse-momentum jet. No excess above the expected backgrounds is observed. Exclusion limits are set at 95% confidence level on the masses of the chargino and gluino for different chargino lifetimes. Chargino masses up to 660 (210) G $$\text {eV}$$ eV are excluded in scenarios where the chargino is a pure wino (higgsino). For charginos produced during the cascade decay of a heavy gluino, gluinos with masses below 2.1 T $$\text {eV}$$ eV are excluded for a chargino mass of 300 G $$\text {eV}$$ eV and a lifetime of 0.2 ns. 

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