More Like this

1. Museum genomics reveals the Xerces blue butterfly ( Glaucopsyche xerces ) was a distinct species driven to extinction

   https://doi.org/10.1098/rsbl.2021.0123  

   Grewe, Felix; Kronforst, Marcus R.; Pierce, Naomi E.; Moreau, Corrie S. (July 2021, Biology Letters)

   null (Ed.)

   The last Xerces blue butterfly was seen in the early 1940s, and its extinction is credited to human urban development. This butterfly has become a North American icon for insect conservation, but some have questioned whether it was truly a distinct species, or simply an isolated population of another living species. To address this question, we leveraged next-generation sequencing using a 93-year-old museum specimen. We applied a genome skimming strategy that aimed for the organellar genome and high-copy fractions of the nuclear genome by a shallow sequencing approach. From these data, we were able to recover over 200 million nucleotides, which assembled into several phylogenetically informative markers and the near-complete mitochondrial genome. From our phylogenetic analyses and haplotype network analysis we conclude that the Xerces blue butterfly was a distinct species driven to extinction. 

   more » « less
2. Study on Dynamic Behaviors of Hypoid Gears Under Variable Tidal Current Energy Harvesting Conditions

   https://doi.org/10.3390/machines13030178  

   Huang, Dequan; Li, Yan; Zheng, Xingyuan; Li, Gang (March 2025, Machines)

   This study investigates dynamic behaviors of hypoid gear rotor systems under variable tidal current energy harvesting conditions through numerical simulations and experimental validation. The study examines dynamic responses of a hypoid gear rotor system induced by cyclical tidal current variations, which generate fluctuating loads and bidirectional rotational speeds in tidal energy conversion systems. Two hypoid gear pairs, modified through precise manufacturing parameters, are evaluated to optimize tooth contact patterns for bidirectional tidal loading conditions. A coupled torsional vibration model is developed, incorporating variable transmission error and mesh stiffness. Experimental validation of dynamic performances of hypoid gear pairs was conducted on a bevel gear testing rig, which can measure both torsional and translational vibrations across diverse tidal speed profiles. The experimental results demonstrate that second-order primary resonances exhibit heightened vibration intensity during flow-reversal phases. This phenomenon has significant implications for system power efficiency and acoustic emissions. The findings extend the current understanding of hypoid gear optimization for tidal energy-harvesting applications. 

   more » « less
3. Multi-Point Control for Face-Milled Spiral Bevel Gears with a Predesigned Fourth-Order Motion Curve

   https://doi.org/10.3390/machines12010034  

   Liu, Yuhui; Chen, Liping; Li, Gang (January 2024, Machines)

   This paper presents an ultimate motion methodology of a face-milling spiral bevel gear pair to synthesize the mating tooth surfaces with a predesigned fourth-order motion curve. The methodology is to control some contact points along the contact path in the process of tooth contact analysis via application of an extended local synthesis which permits some transmission errors rather than zero at the concerned contact point. The modified offset motion correction is selected to demonstrate the proposed methodology. Applied torque corresponding to an elastic approach of 0.00635 mm at the mean contact point is calculated and the loaded tooth contact analysis is performed. Numerical results show that the extended local synthesis can effectively control the transmission errors on the predesigned fourth-order motion curve at arbitrarily predesigned contact points along the contact path of the spiral bevel gear pair. The tooth contact pattern for the actual tooth pair is scattered into three segments since the rotational motion of the driven gear at any instant angular position is dependent on the tooth pair with the least transmission error among the three adjacent tooth pairs. The actual tooth contact patterns of the spiral bevel gear pair become continuous when meshing tooth surfaces are elastically deformed. 

   more » « less
4. High-Quality Genome Assembly and Comprehensive Transcriptome of the Painted Lady Butterfly Vanessa cardui

   https://doi.org/10.1093/gbe/evab145  

   Zhang, Linlin; Steward, Rachel A; Wheat, Christopher W; Reed, Robert D (July 2021, Genome Biology and Evolution)

   Lavrov, Dennis (Ed.)

   Abstract The painted lady butterfly, Vanessa cardui, has the longest migration routes, the widest hostplant diversity, and one of the most complex wing patterns of any insect. Due to minimal culturing requirements, easily characterized wing pattern elements, and technical feasibility of CRISPR/Cas9 genome editing, V. cardui is emerging as a functional genomics model for diverse research programs. Here, we report a high-quality, annotated genome assembly of the V. cardui genome, generated using 84× coverage of PacBio long-read data, which we assembled into 205 contigs with a total length of 425.4 Mb (N50 = 10.3 Mb). The genome was very complete (single-copy complete Benchmarking Universal Single-Copy Orthologs [BUSCO] 97%), with contigs assembled into presumptive chromosomes using synteny analyses. Our annotation used embryonic, larval, and pupal transcriptomes, and 20 transcriptomes across five different wing developmental stages. Gene annotations showed a high level of accuracy and completeness, with 14,437 predicted protein-coding genes. This annotated genome assembly constitutes an important resource for diverse functional genomic studies ranging from the developmental genetic basis of butterfly color pattern, to coevolution with diverse hostplants. 

   more » « less
5. Genome Assembly of the Dogface Butterfly Zerene cesonia

   https://doi.org/10.1093/gbe/evz254  

   Rodriguez-Caro, Luis; Fenner, Jennifer; Benson, Caleb; Van Belleghem, Steven M; Counterman, Brian A; Eyre-Walker, Adam (January 2020, Genome Biology and Evolution)

   Abstract Comparisons of high-quality, reference butterfly, and moth genomes have been instrumental to advancing our understanding of how hybridization, and natural selection drive genomic change during the origin of new species and novel traits. Here, we present a genome assembly of the Southern Dogface butterfly, Zerene cesonia (Pieridae) whose brilliant wing colorations have been implicated in developmental plasticity, hybridization, sexual selection, and speciation. We assembled 266,407,278 bp of the Z. cesonia genome, which accounts for 98.3% of the estimated 271 Mb genome size. Using a hybrid approach involving Chicago libraries with Hi-Rise assembly and a diploid Meraculous assembly, the final haploid genome was assembled. In the final assembly, nearly all autosomes and the Z chromosome were assembled into single scaffolds. The largest 29 scaffolds accounted for 91.4% of the genome assembly, with the remaining ∼8% distributed among another 247 scaffolds and overall N50 of 9.2 Mb. Tissue-specific RNA-seq informed annotations identified 16,442 protein-coding genes, which included 93.2% of the arthropod Benchmarking Universal Single-Copy Orthologs (BUSCO). The Z. cesonia genome assembly had ∼9% identified as repetitive elements, with a transposable element landscape rich in helitrons. Similar to other Lepidoptera genomes, Z. cesonia showed a high conservation of chromosomal synteny. The Z. cesonia assembly provides a high-quality reference for studies of chromosomal arrangements in the Pierid family, as well as for population, phylo, and functional genomic studies of adaptation and speciation. 

   more » « less