skip to main content


Title: Adsorption of Xyloglucan onto Thin Films of Cellulose Nanocrystals and Amorphous Cellulose: Film Thickness Effects
NSF-PAR ID:
10077916
Author(s) / Creator(s):
 ; ; ; ;
Publisher / Repository:
American Chemical Society
Date Published:
Journal Name:
ACS Omega
Volume:
3
Issue:
10
ISSN:
2470-1343
Page Range / eLocation ID:
p. 14004-14012
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. SUMMARY

    Endosidin20 (ES20) is a recently identified cellulose biosynthesis inhibitor (CBI) that targets the catalytic site of plant cellulose synthase (CESA). Here, we screened over 600 ES20 analogs and identified nine active analogs named ES20‐1 to ES20‐9. Among these, endosidin20‐1 (ES20‐1) had stronger inhibitory effects on plant growth and cellulose biosynthesis than ES20. At the biochemical level, we demonstrated that ES20‐1, like ES20, directly interacts with CESA6. At the cellular level, this molecule, like ES20, induced the accumulation of cellulose synthase complexes at the Golgi apparatus and inhibited their secretion to the plasma membrane. Like ES20, ES20‐1 likely targets the catalytic site of CESA. However, through molecular docking analysis using a modeled structure of full‐length CESA6, we found that both ES20 and ES20‐1 might have another target site at the transmembrane regions of CESA6. Besides ES20, other CBIs such as isoxaben, C17, and flupoxam are widely used tools to dissect the mechanism of cellulose biosynthesis and are also valuable resources for the development of herbicides. Here, based on mutant genetic analysis and molecular docking analysis, we have identified the potential target sites of these CBIs on a modeled CESA structure. Some bacteria also produce cellulose, and both ES20 and ES20‐1 inhibited bacterial cellulose biosynthesis. Therefore, we conclude that ES20‐1 is a more potent analog of ES20 that inhibits intrinsic cellulose biosynthesis in plants, and both ES20 and ES20‐1 show an inhibitory effect on bacterial growth and cellulose synthesis, making them excellent tools for exploring the mechanisms of cellulose biosynthesis across kingdoms.

     
    more » « less
  2. Powdered cellulose-reinforced (20 wt%) polypropylene composites were prepared by melt compounding and subsequent injection moldings. We assessed the effect of cellulose reducing ends on the capacity of powdered cellulose to reinforce polypropylene composites after seven days exposure to air circulation during the conditioning of samples. Tensile tests on the composites were performed at 5.08 mm/min. Fourier transform infrared spectroscopy revealed some changes that occurred within the composites by demonstrating a practical decrease in –C=O (1744 cm−1) absorption band intensity. A thermogravimetric analysis indicated differences within the thermal behavior of the prepared composites, showing a higher onset of degradation. Scanning electron microscopy of the fracture areas, together with load–extension curves, further characterized the development of interfacial cellulose/matrix adhesion as well as the brittle and ductile behavior of the composites. The results indicate that the thermal and tensile properties of powdered cellulose/polypropylene are improved by decreasing the amount of cellulose reducing ends in the system. 
    more » « less