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1. Generation of tamoxifen‐inducible Tfap2b‐CreER^T2 mice using CRISPR‐Cas9

   https://doi.org/10.1002/dvg.23582  

   Zhang, Mingyi; Feng, Jifan; Li, Yue; Qin, Peter Z.; Chai, Yang (December 2023, genesis)

   Summary Tfap2b, a pivotal transcription factor, plays critical roles within neural crest cells and their derived lineage. To unravel the intricate lineage dynamics and contribution of these Tfap2b+ cells during craniofacial development, we established aTfap2b‐CreER^T2knock‐in transgenic mouse line using the CRISPR‐Cas9‐mediated homologous direct repair. By breeding with tdTomato reporter mice and initiating Cre activity through tamoxifen induction at distinct developmental time points, we show theTfap2blineage within the key neural crest‐derived domains, such as the facial mesenchyme, midbrain, cerebellum, spinal cord, and limbs. Notably, the migratory neurons stemming from the dorsal root ganglia are visible subsequent to Cre activity initiated at E8.5. Intriguingly, Tfap2b+ cells, serving as the progenitors for limb development, show activity predominantly commencing at E10.5. Across the mouse craniofacial landscape, Tfap2b exhibits a widespread presence throughout the facial organs. Here we validate its role as a marker of progenitors in tooth development and have confirmed that this process initiates from E12.5. Our study not only validates theTfap2b‐CreER^T2transgenic line, but also provides a powerful tool for lineage tracing and genetic targeting ofTfap2b‐expressing cells and their progenitor in a temporally and spatially regulated manner during the intricate process of development and organogenesis. 

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2. Sensitized Photooxidation of Ortho ‐Prenyl Phenol: Biomimetic Dihydrobenzofuran Synthesis and Total ¹O₂ Quenching ^†

   https://doi.org/10.1111/php.13689  

   Jabeen, Shakeela; Ghosh, Goutam; Lapoot, Lloyd; Durantini, Andrés M.; Greer, Alexander (September 2022, Photochemistry and Photobiology)

   Abstract The sensitized photooxidation ofortho‐prenyl phenol is described with evidence that solvent aproticity favors the formation of a dihydrobenzofuran [2‐(prop‐1‐en‐2‐yl)‐2,3‐dihydrobenzofuran], a moiety commonly found in natural products. Benzene solvent increased the total quenching rate constant (k_T) of singlet oxygen with prenyl phenol by ~10‐fold compared to methanol. A mechanism is proposed with preferential addition of singlet oxygen to prenyl site due to hydrogen bonding with the phenol OH group, which causes a divergence away from the singlet oxygen ‘ene’ reaction toward the dihydrobenzofuran as the major product. The reaction is a mixed photooxidized system since an epoxide arises by a type I sensitized photooxidation. 

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3. 5′‐End sequencing in Saccharomyces cerevisiae offers new insights into 5′‐ends of tRNA^{H is} and snoRNAs

   https://doi.org/10.1002/1873-3468.13364  

   Dodbele, Samantha; Moreland, Blythe; Gardner, Spencer M.; Bundschuh, Ralf; Jackman, Jane E. (April 2019, FEBS Letters)
4. Isolation and reactivity of a gold( i ) hydroxytrifluoroborate complex stabilized by anion-π ⁺ interactions

   https://doi.org/10.1039/D1CC04105C  

   Zhou, Jiliang; Litle, Elishua D.; Gabbaï, François P. (October 2021, Chemical Communications)

   A 9,9-dimethylxanthene-based ligand substituted at the 4- and 5-positions by a phosphine and a xanthylium unit, respectively, has been prepared and converted into an AuCl complex, the structure of which reveals an intramolecular Au–Cl⋯π + interaction. This new ligand platform was also found to support the formation of an unprecedented hydroxytrifluoroborate derivative featuring a “hard/soft” mismatched Au– μ (OH)–BF 3 motif. Despite its surprising stability, this gold hydroxytrifluoroborate complex is a remarkably potent carbophilic catalyst which readily activates alkynes, without activator. 

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5. Synthesis, Characterization and Photocleavage of Bis‐decyl Pteroic Acid: A Folate Derivative with Affinity to Biomembranes ^†

   https://doi.org/10.1111/php.13755  

   Fonseca, José Luis; Sosa, María José; Petroselli, Gabriela; Erra‐Balsells, Rosa; Quindt, Matías I.; Bonesi, Sergio M.; Greer, Alexander; Greer, Edyta M.; Thomas, Andrés H.; Vignoni, Mariana (March 2023, Photochemistry and Photobiology)