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1. Accomplishing a N-E-W (nutrient-energy-water) synergy in a bioelectrochemical nitritation-anammox process

   https://doi.org/10.1038/s41598-019-45620-2  

   Ghimire, Umesh ; Gude, Veera Gnaneswar ( June 2019 , Scientific Reports)

   This study reports an investigation of the concept, application and performance of a novel bioelectrochemical nitritation-anammox microbial desalination cell (MDC) for resource-efficient wastewater treatment and desalination. Two configurations of anammox MDCs (anaerobic-anammox cathode MDC (AnA_moxMDC) and nitration-anammox cathode MDC (NiA_moxMDC)) were compared with an air cathode MDC (CMDC), operated in fed-batch mode. Results from this study showed that the maximum power density produced by NiA_moxMDC (1,007 mW/m³) was higher than that of AnA_moxMDC (444 mW/m³) and CMDC (952 mW/m³). More than 92% of ammonium-nitrogen (NH₄⁺-N) removal was achieved in NiA_moxMDC, significantly higher than AnA_moxMDC (84%) and CMDC (77%). The NiA_moxMDC performed better than CMDC and AnA_moxMDC in terms of power density, COD removal and salt removal in desalination chamber. In addition, cyclic voltammetry analysis of anammox cathode showed a redox peak centered at −140 mV Vs Ag/AgCl confirming the catalytic activity of anammox bacteria towards the electron transfer process. Further, net energy balance of the NiA_moxMDC was the highest (NiA_moxMDC-0.022 kWh/m³>CMDC-0.019 kWh/m³>AnA_moxMDC-0.021 kWh/m³) among the three configurations. This study demonstrated, for the first time, a N-E-W synergy for resource-efficient wastewater treatment using nitritation-anammox process.

    

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2. Microbiomes and Planctomycete diversity in large-scale aquaria habitats

   https://doi.org/10.1371/journal.pone.0267881  

   Elbon, Claire E. ; LeCleir, Gary R. ; Tuttle, Matthew J. ; Jurgensen, Sophie K. ; Demas, Thomas G. ; Keller, Christian J. ; Stewart, Tina ; Buchan, Alison ( May 2022 , PLOS ONE)

   Senko, John M. (Ed.)

   In commercial large-scale aquaria, controlling levels of nitrogenous compounds is essential for macrofauna health. Naturally occurring bacteria are capable of transforming toxic nitrogen species into their more benign counterparts and play important roles in maintaining aquaria health. Nitrification, the microbially-mediated transformation of ammonium and nitrite to nitrate, is a common and encouraged process for management of both commercial and home aquaria. A potentially competing microbial process that transforms ammonium and nitrite to dinitrogen gas (anaerobic ammonium oxidation [anammox]) is mediated by some bacteria within the phylum Planctomycetes. Anammox has been harnessed for nitrogen removal during wastewater treatment, as the nitrogenous end product is released into the atmosphere rather than in aqueous discharge. Whether anammox bacteria could be similarly utilized in commercial aquaria is an open question. As a first step in assessing the viability of this practice, we (i) characterized microbial communities from water and sand filtration systems for four habitats at the Tennessee Aquarium and (ii) examined the abundance and anammox potential of Planctomycetes using culture-independent approaches. 16S rRNA gene amplicon sequencing revealed distinct, yet stable, microbial communities and the presence of Planctomycetes (~1–15% of library reads) in all sampled habitats. Preliminary metagenomic analyses identified the genetic potential for multiple complete nitrogen metabolism pathways. However, no known genes diagnostic for the anammox reaction were found in this survey. To better understand the diversity of this group of bacteria in these systems, a targeted Planctomycete-specific 16S rRNA gene-based PCR approach was used. This effort recovered amplicons that share <95% 16S rRNA gene sequence identity to previously characterized Planctomycetes, suggesting novel strains within this phylum reside within aquaria.

    

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3. Primary sludge fermentate as carbon source for mainstream partial denitrification–anammox (PdNA)

   https://doi.org/10.1002/wer.1492  

   Ali, Priyanka ; Zalivina, Nadezhda ; Le, Tri ; Riffat, Rumana ; Ergas, Sarina ; Wett, Bernhard ; Murthy, Sudhir ; Al‐Omari, Ahmed ; deBarbadillo, Christine ; Bott, Charles ; et al ( January 2021 , Water Environment Research)

   Primary sludge fermentate, a concentrated hydrolyzed wastewater carbon, was evaluated for use as an alternative carbon source for mainstream partial denitrification–anammox (PdNA) in a suspended growth activated sludge process in terms of partial denitrification (PdN) efficiency, PdNA nitrogen removal contributions, and final effluent quality. Fermenter operation at a 2‐day sludge retention time (SRT) resulted in the maximum achievable yield of 0.14 ± 0.05 g sCOD/g VSS without release of excessive ammonia and phosphorus to the system. Based on the results of batch experiments, fermentate addition led to PdN efficiency of 93 ± 14%, which was similar to acetate at a nitrate residual of 2–3 mg N/L. In the pilot‐scale mainstream deammonification reactor, PdN efficiency using fermentate was 49 ± 24%, which was lower than acetate (66 ± 24% during acetate period I and 70 ± 21% during acetate period II), most probably due to lower nitrate and ammonium kinetics in the PdN zone. Methanol cost‐saving potential for the application of PdNA as the main short‐cut nitrogen pathway was estimated to be 30% to 55% depending on the PdN efficiency achieved.

   Primary sludge fermentate was evaluated as an alternative carbon source for mainstream partial denitrification–anammox (PdNA).

   Fermenter operated at a 1 to 2 day SRT resulted in the maximum achievable yield without the release of excessive ammonia and phosphorus to the system.

   Although 93% partial denitrification efficiency was achieved with fermentate in batch experiments, around 49% PdN efficiency was achieved in pilot studies.

   Application of PdNA with fermentate can result in significant methanol cost savings.

    

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4. Toxicity of azoles towards the anaerobic ammonium oxidation (anammox) process

   https://doi.org/10.1002/jctb.6285  

   Lakhey, Nivrutti ; Li, Guangbin ; Sierra‐Alvarez, Reyes ; Field, Jim A. ( December 2019 , Journal of Chemical Technology & Biotechnology)

   Azoles are an important class of compounds that are widely used as corrosion inhibitors in aircraft de‐icing agents, cooling towers, semiconductor manufacturing and household dishwashing detergents. They also are important moieties in pharmaceutical drugs and fungicides. Azoles are widespread emerging contaminants occurring frequently in water bodies. Azole compounds can potentially cause inhibition towards key biological processes in natural ecosystems and wastewater treatment processes. Of particular concern is the inhibition of azoles to the nitrification process (aerobic oxidation of ammonium). This study investigated the acute toxicity of azole compounds towards the anaerobic ammonia oxidation (anammox) process, which is an important environmental biotechnology gaining traction for nutrient‐nitrogen removal during wastewater treatment. In this study, using batch bioassay techniques, the anammox toxicity of eight commonly occurring azole compounds was evaluated.

   The results show that 1H‐benzotriazole and 5‐methyl‐1H‐benzotriazole had the highest inhibitory effect on the anammox process, causing 50% decrease in anammox activity (IC₅₀) at concentrations of 19.6 and 17.8 mg L⁻¹, respectively. 1H‐imidazole caused less severe toxicity with an IC₅₀of 79.4 mg L⁻¹. The other azole compounds were either nontoxic (1H‐pyrazole, 1H‐1,2,4‐triazole and 1‐methyl‐pyrazole) or at best mildly toxic (1H‐benzotriazole‐5‐carboxylic acid and 3,5‐dimethyl‐1H‐pyrazole) towards the anammox bacteria at the concentrations tested.

   This study showed that most azole compounds tested displayed mild to low or no toxicity towards the anammox bacteria. The anammox bacteria were found to be far less sensitive to azoles compared to nitrifying bacteria. © 2019 Society of Chemical Industry

    

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5. Fate and Transport of the Biologically Treated Landfill Leachate Induced Dissolved Organic Nitrogen (DON)

   Md Ashik Ahmed*, Md Redowan ( June 2023 , AEESP Research and Education Conference, Northeastern University, June 20-23, 2023)

   This study evaluated the performance of sequencing batch reactors (SBR) in the fate and transport of dissolved organic nitrogen (DON) using a blend of wastewater and landfill leachate. Most nitrogen removal methods concentrate on inorganic nitrogen, whereas some biological processes add DON to the effluent. Two reactors were introduced with wastewater and landfill leachate of high and low organic carbon and compared them to a reactor without leachate. DON transformation, characterization, and microbial community dispersion were examined to understand the effects of leachate-induced effluent DON on the biological nitrogen removal process. The ammonium removal efficiencies were found 96, 97, and 98%; COD removal efficiencies were 75, 59, and 63%; and total nitrogen (TN) removal efficiencies were 83, 86, and 88%, for R1, R2, and R3, respectively. The effluent nitrate concentrations were found 1.67 ± 0.89 (R1), 3.05 ± 2.08 (R2), and 1.31 ± 1.30 (R3) mg/L and DON went down from 9.67 ± 2.5 to 6.02 ± 2.8 mg/L (R1), 9.29 ± 3.4 to 7.49 ± 3.6 mg/L (R2), and 3.59 ± 1.6 to 2.08 ± 1.1 mg/L (R3). Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) and excitation-emission matrices (EEMs) with parallel factor (PARAFAC) analysis were used to characterize DON. Microbial community analysis was also conducted. Leachate-induced DON discharge's environmental effects were assessed using in-situ aquatic ecosystem algal bioassay. SBR system removed most inorganic nitrogen species and a small amount of leachate-induced DON. The study emphasizes the need for independent investigations to assess their effects on receiving water bodies. 

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