- Award ID(s):
- 1508070
- NSF-PAR ID:
- 10097206
- Date Published:
- Journal Name:
- Tetrahedron Letters
- Volume:
- 57
- Issue:
- 3
- ISSN:
- 0040-4039
- Page Range / eLocation ID:
- 415 to 419
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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Summary LIKE HETEROCHROMATIN PROTEIN1 (LHP1) encodes the only plant homologue of the metazoan HETEROCHROMATIN PROTEIN1 (HP1) protein family. The LHP1 protein is necessary for proper epigenetic regulation of a range of developmental processes in plants. LHP1 is a transcriptional repressor of flowering‐related genes, such as
FLOWERING LOCUS T (FT ),FLOWERING LOCUS C (FLC ),AGAMOUS (AG ) andAPETALA 3 (AP3 ). We found that LHP1 interacts with importin α‐1 (IMPα‐1), importin α‐2 (IMPα‐2) and importin α‐3 (IMPα‐3) bothin vitro andin vivo . A genetic approach revealed that triple mutation ofimpα‐1 ,impα‐2 andimpα‐3 resulted in Arabidopsis plants with a rapid flowering phenotype similar to that of plants with mutations inlhp1 due to the upregulation ofFT expression. Nuclear targeting of LHP1 was severely impaired in theimpα triple mutant, resulting in the de‐repression of LHP1 target genesAG ,AP3 andSHATTERPROOF 1 as well asFT . Therefore, the importin proteins IMPα‐1, ‐2 and ‐3 are necessary for the nuclear import of LHP1.