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Title: A single-flask synthesis of α-alkylidene and α-benzylidene lactones from ethoxyacetylene, epoxides/oxetanes, and carbonyl compounds
Award ID(s):
1508070
NSF-PAR ID:
10097206
Author(s) / Creator(s):
; ;
Date Published:
Journal Name:
Tetrahedron Letters
Volume:
57
Issue:
3
ISSN:
0040-4039
Page Range / eLocation ID:
415 to 419
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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  1. Summary

    LIKE HETEROCHROMATIN PROTEIN1 (LHP1) encodes the only plant homologue of the metazoan HETEROCHROMATIN PROTEIN1 (HP1) protein family. The LHP1 protein is necessary for proper epigenetic regulation of a range of developmental processes in plants. LHP1 is a transcriptional repressor of flowering‐related genes, such asFLOWERING LOCUS T(FT),FLOWERING LOCUS C(FLC),AGAMOUS(AG) andAPETALA 3(AP3). We found that LHP1 interacts with importin α‐1 (IMPα‐1), importin α‐2 (IMPα‐2) and importin α‐3 (IMPα‐3) bothin vitroandin vivo. A genetic approach revealed that triple mutation ofimpα‐1,impα‐2andimpα‐3resulted in Arabidopsis plants with a rapid flowering phenotype similar to that of plants with mutations inlhp1due to the upregulation ofFTexpression. Nuclear targeting of LHP1 was severely impaired in theimpαtriple mutant, resulting in the de‐repression of LHP1 target genesAG,AP3andSHATTERPROOF 1as well asFT. Therefore, the importin proteins IMPα‐1, ‐2 and ‐3 are necessary for the nuclear import of LHP1.

     
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