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Title: Bright-field holography: cross-modality deep learning enables snapshot 3D imaging with bright-field contrast using a single hologram
Abstract

Digital holographic microscopy enables the 3D reconstruction of volumetric samples from a single-snapshot hologram. However, unlike a conventional bright-field microscopy image, the quality of holographic reconstructions is compromised by interference fringes as a result of twin images and out-of-plane objects. Here, we demonstrate that cross-modality deep learning using a generative adversarial network (GAN) can endow holographic images of a sample volume with bright-field microscopy contrast, combining the volumetric imaging capability of holography with the speckle- and artifact-free image contrast of incoherent bright-field microscopy. We illustrate the performance of this “bright-field holography” method through the snapshot imaging of bioaerosols distributed in 3D, matching the artifact-free image contrast and axial sectioning performance of a high-NA bright-field microscope. This data-driven deep-learning-based imaging method bridges the contrast gap between coherent and incoherent imaging, and enables the snapshot 3D imaging of objects with bright-field contrast from a single hologram, benefiting from the wave-propagation framework of holography.

 
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PAR ID:
10153484
Author(s) / Creator(s):
; ; ; ; ; ;
Publisher / Repository:
Nature Publishing Group
Date Published:
Journal Name:
Light: Science & Applications
Volume:
8
Issue:
1
ISSN:
2047-7538
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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