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Foreground mitigation is critical to all next-generation radio interferometers that target cosmology using the redshifted neutral hydrogen 21 cm emission line. Attempts to remove this foreground emission have led to new analysis techniques as well as new developments in hardware specifically dedicated to instrument beam and gain calibration, including stabilized signal injection into the interferometric array and drone-based platforms for beam mapping. The radio calibration sources currently used in the literature are broad-band incoherent sources that can only be detected as excess power and with no direct sensitivity to phase information. In this paper, we describe a digital radio source which uses Global Positioning Satellite (GPS) derived time stamps to form a deterministic signal that can be broadcast from an aerial platform. A copy of this source can be deployed locally at the instrument correlator such that the received signal from the aerial platform can be correlated with the local copy, and the resulting correlation can be measured in both amplitude and phase for each interferometric element. We define the requirements for such a source, describe an initial implementation and verification of this source using commercial Software Defined Radio boards, and present beam map slices from antenna range measurements using the commercial boards. We found that the commercial board did not meet all requirements, so we also suggest future directions using a more sophisticated chipset.
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High-speed device synchronization in optical microscopy with an open-source hardware control platform
Abstract Azimuthal beam scanning eliminates the uneven excitation field arising from laser interference in through-objective total internal reflection fluorescence (TIRF) microscopy. The same principle can be applied to scanning angle interference microscopy (SAIM), where precision control of the scanned laser beam presents unique technical challenges for the builders of custom azimuthal scanning microscopes. Accurate synchronization between the instrument computer, beam scanning system and excitation source is required to collect high quality data and minimize sample damage in SAIM acquisitions. Drawing inspiration from open-source prototyping systems, like the Arduino microcontroller boards, we developed a new instrument control platform to be affordable, easily programmed, and broadly useful, but with integrated, precision analog circuitry and optimized firmware routines tailored to advanced microscopy. We show how the integration of waveform generation, multiplexed analog outputs, and native hardware triggers into a single central hub provides a versatile platform for performing fast circle-scanning acquisitions, including azimuthal scanning SAIM and multiangle TIRF. We also demonstrate how the low communication latency of our hardware platform can reduce image intensity and reconstruction artifacts arising from synchronization errors produced by software control. Our complete platform, including hardware design, firmware, API, and software, is available online for community-based development and collaboration.
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- Award ID(s):
- 1752226
- PAR ID:
- 10153657
- Publisher / Repository:
- Nature Publishing Group
- Date Published:
- Journal Name:
- Scientific Reports
- Volume:
- 9
- Issue:
- 1
- ISSN:
- 2045-2322
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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