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Title: High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato
Abstract

CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potatoU6promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the ArabidopsisU6promotor, driving expression of the CRISPR component, with endogenous potatoU6promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.

Authors:
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Publication Date:
NSF-PAR ID:
10153863
Journal Name:
Scientific Reports
Volume:
9
Issue:
1
ISSN:
2045-2322
Publisher:
Nature Publishing Group
Sponsoring Org:
National Science Foundation
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