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Title: Aminopolycarboxylates by the Periplasmic EDTA- Binding Protein EppA from Chelativorans sp. BNC1
The widespread use of synthetic aminopolycarboxylates, such as ethylenediaminetetraacetate (EDTA), as chelating agents has led to their contamination in the environment as stable metal–chelate complexes. Microorganisms can transport free EDTA, but not metal–EDTA complexes, into cells for metabolism. An ABC-type transporter for free EDTA uptake in Chelativorans sp. BNC1 was investigated to understand the mechanism of the ligand selectivity. We solved the X-ray crystal structure of the periplasmic EDTA-binding protein (EppA) and analyzed its structure–function relations through isothermal titration calorimetry, site-directed mutagenesis, molecular docking, and quantum chemical analysis. EppA had high affinities for EDTA and other aminopolycarboxylates, which agrees with structural analysis, showing that its binding pocket could accommodate free aminopolycarboxylates. Further, key amino acid residues involved in the binding were identified. Our results suggest that EppA is a general binding protein for the uptake of free aminopolycarboxylates. This finding suggests that bacterial cells import free aminopolycarboxylates, explaining why stable metal–chelate complexes are resistant to degradation, as they are not transported into the cells for degradation.  more » « less
Award ID(s):
1804699
PAR ID:
10157186
Author(s) / Creator(s):
; ; ; ; ;
Date Published:
Journal Name:
International journal of molecular sciences
Volume:
21
ISSN:
1661-6596
Page Range / eLocation ID:
3940
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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