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Abstract Nucleic acid detection plays a crucial role in various applications, including disease diagnostics, research development, food safety, and environmental health monitoring. A rapid, point‐of‐care (POC) nucleic acid test can greatly benefit healthcare system by providing timely diagnosis for effective treatment and patient management, as well as supporting diseases surveillance for emerging pandemic diseases. Recent advancements in nucleic acids technology have led to rapid assays for single‐stranded nucleic acids that can be integrated into simple and miniaturized platforms for ease of use. In this review, the study focuses on the developments in isothermal amplification, nucleic acid hybridization circuits, various enzyme‐based signal reporting mechanisms, and detection platforms that show promise for POC testing. The study also evaluates critical technical breakthroughs to identify the advantages and disadvantages of these methods in various applications.
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Integrated microfluidic systems with sample preparation and nucleic acid amplification
Rapid, efficient and accurate nucleic acid molecule detection is important in the screening of diseases and pathogens, yet remains a limiting factor at point of care (POC) treatment. Microfluidic systems are characterized by fast, integrated, miniaturized features which provide an effective platform for qualitative and quantitative detection of nucleic acid molecules. The nucleic acid detection process mainly includes sample preparation and target molecule amplification. Given the advancements in theoretical research and technological innovations to date, nucleic acid extraction and amplification integrated with microfluidic systems has advanced rapidly. The primary goal of this review is to outline current approaches used for nucleic acid detection in the context of microfluidic systems. The secondary goal is to identify new approaches that will help shape future trends at the intersection of nucleic acid detection and microfluidics, particularly with regard to increasing disease and pathogen detection for improved diagnosis and treatment.
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- Award ID(s):
- 1709160
- PAR ID:
- 10161122
- Date Published:
- Journal Name:
- Lab on a Chip
- Volume:
- 19
- Issue:
- 17
- ISSN:
- 1473-0197
- Page Range / eLocation ID:
- 2769 to 2785
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1039/c9lc00389d
