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Title: Biodynamic digital holographic speckle microscopy for oocyte and embryo metabolic evaluation

Assisted reproductive technologies seek to improve the success rate of pregnancies. Morphology scoring is a common approach to evaluate oocyte and embryo viability prior to embryo transferin utero, but the efficacy of the method is low. We apply biodynamic imaging, based on dynamic light scattering and low-coherence digital holography, to assess the metabolic activity of oocytes and embryos. A biodynamic microscope, developed to image small and translucent biological specimens, is inserted into the bay of a commercial inverted microscope that can switch between conventional microscopy channels and biodynamic microscopy. We find intracellular Doppler spectral features that act as noninvasive proxies for embryo metabolic activity that may relate to embryo viability.

 
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Award ID(s):
1911357
NSF-PAR ID:
10201897
Author(s) / Creator(s):
; ; ; ; ;
Publisher / Repository:
Optical Society of America
Date Published:
Journal Name:
Applied Optics
Volume:
60
Issue:
4
ISSN:
1559-128X; APOPAI
Page Range / eLocation ID:
Article No. A222
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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    Is the combined use of fluorescence lifetime imaging microscopy (FLIM)-based metabolic imaging and second harmonic generation (SHG) spindle imaging a feasible and safe approach for noninvasive embryo assessment?

    SUMMARY ANSWER

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    WHAT IS KNOWN ALREADY

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    MAIN RESULTS AND THE ROLE OF CHANCE

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    STUDY FUNDING/COMPETING INTEREST(S)

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