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1. Majority sensing in synthetic microbial consortia

   https://doi.org/10.1038/s41467-020-17475-z  

   Alnahhas, Razan N. ; Sadeghpour, Mehdi ; Chen, Ye ; Frey, Alexis A. ; Ott, William ; Josić, Krešimir ; Bennett, Matthew R. ( July 2020 , Nature Communications)

   As synthetic biocircuits become more complex, distributing computations within multi-strain microbial consortia becomes increasingly beneficial. However, designing distributed circuits that respond predictably to variation in consortium composition remains a challenge. Here we develop a two-strain gene circuit that senses and responds to which strain is in the majority. This involves a co-repressive system in which each strain produces a signaling molecule that signals the other strain to down-regulate production of its own, orthogonal signaling molecule. This co-repressive consortium links gene expression to ratio of the strains rather than population size. Further, we control the cross-over point for majority viamore »external induction. We elucidate the mechanisms driving these dynamics by developing a mathematical model that captures consortia response as strain fractions and external induction are varied. These results show that simple gene circuits can be used within multicellular synthetic systems to sense and respond to the state of the population.

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2. Mid-Miocene to Present Upper-Plate Deformation of the Southern Cascadia Forearc: Effects of the Superposition of Subduction and Transform Tectonics

   https://doi.org/10.3389/feart.2022.832515  

   McKenzie, Kirsty A. ; Furlong, Kevin P. ; Kirby, Eric ( February 2022 , Frontiers in Earth Science)

   The southern Cascadia forearc undergoes a three-stage tectonic evolution, each stage involving different combinations of tectonic drivers, that produce differences in the upper-plate deformation style. These drivers include subduction, the northward migration of the Mendocino triple junction and associated thickening and thinning related to the Mendocino Crustal Conveyor (MCC) effect, and the NNW translation of the Sierra Nevada-Great Valley (SNGV) block. We combine geodetic data, plate reconstructions, seismic tomography and topographic observations to determine how the southern Cascadia upper plate is deforming in response to the combined effects of subduction and NNW-directed (MCC- and SNGV-related) tectonic processes. The location ofmore »the terrane boundaries between the relatively weak Franciscan complex and the stronger Klamath Mountain province (KMP) and SNGV block has been a key control on the style of upper-plate deformation in the southern Cascadia forearc since the mid-Miocene. At ∼15 Ma, present-day southern Cascadia was in central Cascadia and deformation there was principally controlled by subduction processes. Since ∼5 Ma, this region of the Cascadia upper plate, where the KMP lies inboard of the Franciscan complex, has been deforming in response to both subduction and MCC- and SNGV-related effects. GPS data show that the KMP is currently moving to the NNW at ∼8–12 mm/yr with little internal deformation, largely in response to the northward push of the SNGV block at its southern boundary. In contrast, the Franciscan complex is accommodating high NNW-directed and NE-directed shortening strain produced by MCC-related shortening and subduction coupling respectively. This composite tectonic regime can explain the style of faulting within and west of the KMP. Associated with this Mendocino Crustal Conveyor crustal thickening, seismic tomography imagery shows a region of low velocity material that we interpret to represent crustal flow and injection of Franciscan crust into the KMP at intracrustal levels. We suggest that this MCC-related crustal flow and injection of material into the KMP is a relatively young feature (post ∼5 Ma) and is driving a rejuvenated period of rock uplift within the KMP. This scenario provides a potential explanation for steep channels and high relief, suggestive of rapid erosion rates within the interior of the KMP.« less
3. 3D printed graphene-based self-powered strain sensors for smart tires in autonomous vehicles

   https://doi.org/10.1038/s41467-020-19088-y  

   Maurya, Deepam ; Khaleghian, Seyedmeysam ; Sriramdas, Rammohan ; Kumar, Prashant ; Kishore, Ravi Anant ; Kang, Min Gyu ; Kumar, Vireshwar ; Song, Hyun-Cheol ; Lee, Seul-Yi ; Yan, Yongke ; et al ( October 2020 , Nature Communications)

   The transition of autonomous vehicles into fleets requires an advanced control system design that relies on continuous feedback from the tires. Smart tires enable continuous monitoring of dynamic parameters by combining strain sensing with traditional tire functions. Here, we provide breakthrough in this direction by demonstrating tire-integrated system that combines direct mask-less 3D printed strain gauges, flexible piezoelectric energy harvester for powering the sensors and secure wireless data transfer electronics, and machine learning for predictive data analysis. Ink of graphene based material was designed to directly print strain sensor for measuring tire-road interactions under varying driving speeds, normal load,more »and tire pressure. A secure wireless data transfer hardware powered by a piezoelectric patch is implemented to demonstrate self-powered sensing and wireless communication capability. Combined, this study significantly advances the design and fabrication of cost-effective smart tires by demonstrating practical self-powered wireless strain sensing capability.

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4. Phage Infection Restores PQS Signaling and Enhances Growth of a Pseudomonas aeruginosa lasI Quorum-Sensing Mutant

   https://doi.org/10.1128/jb.00557-21  

   Høyland-Kroghsbo, Nina Molin ; Bassler, Bonnie L. ( January 2022 , Journal of Bacteriology)

   Bondy-Denomy, Joseph (Ed.)

   ABSTRACT Chemical communication between bacteria and between bacteria and the bacteriophage (phage) viruses that prey on them can shape the outcomes of phage-bacterial encounters. Quorum sensing (QS) is a bacterial cell-to-cell communication process that promotes collective undertaking of group behaviors. QS relies on the production, release, accumulation, and detection of signal molecules called autoinducers. Phages can exploit QS-mediated communication to manipulate their hosts and maximize their own survival. In the opportunistic pathogen Pseudomonas aeruginosa , the LasI/R QS system induces the RhlI/R QS system, and in opposing manners, these two systems control the QS system that relies on the autoinducermore »called PQS. A P. aeruginosa Δ lasI mutant is impaired in PQS synthesis, leading to accumulation of the precursor molecule HHQ, and HHQ suppresses growth of the P. aeruginosa Δ lasI strain. We show that, in response to a phage infection, the P. aeruginosa Δ lasI mutant reactivates QS, which, in turn, restores pqsH expression, enabling conversion of HHQ into PQS. Moreover, downstream QS target genes encoding virulence factors are induced. Additionally, phage-infected P. aeruginosa Δ lasI cells transiently exhibit superior growth compared to uninfected cells. IMPORTANCE Clinical isolates of P. aeruginosa frequently harbor mutations in particular QS genes. Here, we show that infection by select temperate phages restores QS, a cell-to-cell communication mechanism in a P. aeruginosa QS mutant. Restoration of QS increases expression of genes encoding virulence factors. Thus, phage infection of select P. aeruginosa strains may increase bacterial pathogenicity, underscoring the importance of characterizing phage-host interactions in the context of bacterial mutants that are relevant in clinical settings.« less
5. Floquet engineering of optical lattices with spatial features and periodicity below the diffraction limit

   https://doi.org/10.1088/1367-2630/ab500f  

   Subhankar, S. ; Bienias, P. ; Titum, P. ; Tsui, T-C ; Wang, Y. ; Gorshkov, A. V. ; Rolston, S. L. ; Porto, J. V. ( November 2019 , New Journal of Physics)

   Floquet engineering or coherent time-periodic driving of quantum systems has been successfully used to synthesize Hamiltonians with novel properties. In ultracold atomic systems, this has led to experimental realizations of artificial gauge fields, topological bandstructures, and observation of dynamical localization, to name a few. Here we present a Floquet-based framework to stroboscopically engineer Hamiltonians with spatial features and periodicity below the diffraction limit of light used to create them by time-averaging over various configurations of a 1D optical Kronig–Penney (KP) lattice. The KP potential is a lattice of narrow subwavelength barriers spaced by half the optical wavelength (λ/2) andmore »arises from the nonlinear optical response of the atomic dark state. Stroboscopic control over the strength and position of this lattice requires time-dependent adiabatic manipulation of the dark-state spin composition. We investigate adiabaticity requirements and shape our time-dependent light fields to respect the requirements. We apply this framework to show that aλ/4-spaced lattice can be synthesized using realistic experimental parameters as an example, discuss mechanisms that limit lifetimes in these lattices, explore candidate systems and their limitations, and treat adiabatic loading into the ground band of these lattices.

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