Optical diffraction tomography (ODT) is a label-free and noninvasive technique for biological imaging. However, ODT is only applicable to weakly scattering objects. To extend ODT to the multiple-scattering regime, more advanced inversion algorithms have been developed, including optimization-based ODT (Opti-ODT) and iterative ODT (iODT). In this paper, we propose a combined strategy, namely, an iODT initialization for Opti-ODT, based on the observed complementarity of their individual advantages. This study numerically demonstrates that under this combined strategy, the reconstruction can accurately converge to a better local minimum, especially in the case of multiply scattering objects with large optical path differences.
Iterative optical diffraction tomography for illumination scanning configuration
Optical diffraction tomography (ODT) is used to reconstruct refractive-index distributions from multiple measurements in the object rotating configuration (ORC) or the illumination scanning configuration (ISC). Because of its fast data acquisition and stability, ISC-based ODT has been widely used for biological imaging. ODT typically fails to reconstruct multiply-scattering samples. The previously developed iterative ODT (iODT) was for the multiply-scattering objects in ORC, and could not be directly applied to ISC. To resolve this mismatch, we developed an ISC update and numerically demonstrated its accuracy. With the same prior knowledge, iODT-ISC outperforms conventional ODT in resolving the missing-angle problem.
more » « less- NSF-PAR ID:
- 10206177
- Publisher / Repository:
- Optical Society of America
- Date Published:
- Journal Name:
- Optics Express
- Volume:
- 28
- Issue:
- 26
- ISSN:
- 1094-4087; OPEXFF
- Format(s):
- Medium: X Size: Article No. 39904
- Size(s):
- Article No. 39904
- Sponsoring Org:
- National Science Foundation
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