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  • Improved reference genome for Cyclotella cryptica CCMP332, a model for cell wall morphogenesis, salinity adaptation, and lipid production in diatoms (Bacillariophyta)
Title: Improved reference genome for Cyclotella cryptica CCMP332, a model for cell wall morphogenesis, salinity adaptation, and lipid production in diatoms (Bacillariophyta)
Abstract The diatom, Cyclotella cryptica, is a well-established model species for physiological studies and biotechnology applications of diatoms. To further facilitate its use as a model diatom, we report an improved reference genome assembly and annotation for C. cryptica strain CCMP332. We used a combination of long- and short-read sequencing to assemble a high-quality and contaminant-free genome. The genome is 171 Mb in size and consists of 662 scaffolds with a scaffold N50 of 494 kb. This represents a 176-fold decrease in scaffold number and 41-fold increase in scaffold N50 compared to the previous assembly. The genome contains 21,250 predicted genes, 75% of which were assigned putative functions. Repetitive DNA comprises 59% of the genome, and an improved classification of repetitive elements indicated that a historically steady accumulation of transposable elements has contributed to the relatively large size of the C. cryptica genome. The high-quality C. cryptica genome will serve as a valuable reference for ecological, genetic, and biotechnology studies of diatoms.  more » « less
Award ID(s):
1651087
PAR ID:
10231024
Author(s) / Creator(s):
; ; ; ;
Publisher / Repository:
OUP
Date Published:
Journal Name:
G3 Genes|Genomes|Genetics
Volume:
10
Issue:
9
ISSN:
2160-1836
Page Range / eLocation ID:
2965 to 2974
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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