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Title: Compositional and genetic alterations in Graves’ disease gut microbiome reveal specific diagnostic biomarkers
Abstract

Graves’ Disease is the most common organ-specific autoimmune disease and has been linked in small pilot studies to taxonomic markers within the gut microbiome. Important limitations of this work include small sample sizes and low-resolution taxonomic markers. Accordingly, we studied 162 gut microbiomes of mild and severe Graves’ disease (GD) patients and healthy controls. Taxonomic and functional analyses based on metagenome-assembled genomes (MAGs) and MAG-annotated genes, together with predicted metabolic functions and metabolite profiles, revealed a well-defined network of MAGs, genes and clinical indexes separating healthy from GD subjects. A supervised classification model identified a combination of biomarkers including microbial species, MAGs, genes and SNPs, with predictive power superior to models from any single biomarker type (AUC = 0.98). Global, cross-disease multi-cohort analysis of gut microbiomes revealed high specificity of these GD biomarkers, notably discriminating against Parkinson’s Disease, and suggesting that non-invasive stool-based diagnostics will be useful for these diseases.

 
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Award ID(s):
2038509
NSF-PAR ID:
10233484
Author(s) / Creator(s):
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Publisher / Repository:
Oxford University Press
Date Published:
Journal Name:
The ISME Journal
Volume:
15
Issue:
11
ISSN:
1751-7362
Format(s):
Medium: X Size: p. 3399-3411
Size(s):
["p. 3399-3411"]
Sponsoring Org:
National Science Foundation
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