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Title: ¡Viva la mitochondria!: harnessing yeast mitochondria for chemical production
ABSTRACT The mitochondria, often referred to as the powerhouse of the cell, offer a unique physicochemical environment enriched with a distinct set of enzymes, metabolites and cofactors ready to be exploited for metabolic engineering. In this review, we discuss how the mitochondrion has been engineered in the traditional sense of metabolic engineering or completely bypassed for chemical production. We then describe the more recent approach of harnessing the mitochondria to compartmentalize engineered metabolic pathways, including for the production of alcohols, terpenoids, sterols, organic acids and other valuable products. We explain the different mechanisms by which mitochondrial compartmentalization benefits engineered metabolic pathways to boost chemical production. Finally, we discuss the key challenges that need to be overcome to expand the applicability of mitochondrial engineering and reach the full potential of this emerging field.  more » « less
Award ID(s):
1751840
PAR ID:
10285253
Author(s) / Creator(s):
; ;
Date Published:
Journal Name:
FEMS Yeast Research
Volume:
20
Issue:
6
ISSN:
1567-1356
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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