Plant ‐specific lysin‐motif receptor‐like kinases (LysM‐RLKs) are implicated in the perception of We tested Compared with wild‐type plants, MtLYK9 thus has a dual role in plant immunity and the AM symbiosis, which raises questions about the functioning and the ancestral origins of such a receptor protein.
During meiosis, crossovers (COs) are typically required to ensure faithful chromosomal segregation. Despite the requirement for at least one CO between each pair of chromosomes, closely spaced double COs are usually underrepresented due to a phenomenon called CO interference. Like
- NSF-PAR ID:
- 10305774
- Publisher / Repository:
- Proceedings of the National Academy of Sciences
- Date Published:
- Journal Name:
- Proceedings of the National Academy of Sciences
- Volume:
- 118
- Issue:
- 47
- ISSN:
- 0027-8424
- Page Range / eLocation ID:
- Article No. e2107543118
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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Summary N ‐acetyl glucosamine‐containing compounds, some of which are important signal molecules in plant−microbe interactions. Among these, both lipo‐chitooligosaccharides (LCOs) and chitooligosaccharides (COs) are proposed as arbuscular mycorrhizal (AM) fungal symbiotic signals. COs can also activate plant defence, although there are scarce data about CO production by pathogens, especially nonfungal pathogens.Medicago truncatula mutants in the LysM‐RLK MtLYK9 for their abilities to interact with the AM fungusRhizophagus irregularis and the oomycete pathogenAphanomyces euteiches . This prompted us to analyse whetherA. euteiches can produce COs.Mtlyk9 mutants had fewer infection events and were less colonised by the AM fungus. By contrast,Mtlyk9 mutants were more heavily infected byA. euteiches and showed more disease symptoms.Aphanomyces euteiches was also shown to produce short COs, mainly CO II, but also CO III and CO IV, and traces of CO V, bothex planta andin planta . -
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We present Atacama Large Millimeter/submillimeter Array (ALMA) sub-kiloparsec- to kiloparsec-scale resolution observations of the [C
II ], CO (9–8), and OH+(11–01) lines along with their dust continuum emission toward the far-infrared (FIR) luminous quasar SDSS J231038.88+185519.7 atz = 6.0031, to study the interstellar medium distribution, the gas kinematics, and the quasar-host system dynamics. We decompose the intensity maps of the [CII ] and CO (9–8) lines and the dust continuum with two-dimensional elliptical Sérsic models. The [CII ] brightness follows a flat distribution with a Sérsic index of 0.59. The CO (9–8) line and the dust continuum can be fit with an unresolved nuclear component and an extended Sérsic component with a Sérsic index of ∼1, which may correspond to the emission from an active galactic nucleus dusty molecular torus and a quasar host galaxy, respectively. The different [CII ] spatial distribution may be due to the effect of the high dust opacity, which increases the FIR background radiation on the [CII ] line, especially in the galaxy center, significantly suppressing the [CII ] emission profile. The dust temperature drops with distance from the center. The effective radius of the dust continuum is smaller than that of the line emission and the dust mass surface density, but is consistent with that of the star formation rate surface density. This may indicate that the dust emission is a less robust tracer of the dust and gas distribution but is a decent tracer of the obscured star formation activity. The OH+(11–01) line shows a P-Cygni profile with an absorption at ∼–400 km s−1, which may indicate an outflow with a neutral gas mass of (6.2 ± 1.2)×108M ⊙along the line of sight. We employed a three-dimensional tilted ring model to fit the [CII ] and CO (9–8) data cubes. The two lines are both rotation dominated and trace identical disk geometries and gas motions. This suggest that the [CII ] and CO (9–8) gas are coplanar and corotating in this quasar host galaxy. The consistent circular velocities measured with [CII ] and CO (9–8) lines indicate that these two lines trace a similar gravitational potential. We decompose the circular rotation curve measured from the kinematic model fit to the [CII ] line into four matter components (black hole, stars, gas, and dark matter). The quasar-starburst system is dominated by baryonic matter inside the central few kiloparsecs. We constrain the black hole mass to be 2.97+0.51-0.77 × 109M ⊙; this is the first time that the dynamical mass of a black hole has been measured atz ∼ 6. This mass is consistent with that determined using the scaling relations from quasar emission lines. A massive stellar component (on the order of 109M ⊙) may have already existed when the Universe was only ∼0.93 Gyr old. The relations between the black hole mass and the baryonic mass of this quasar indicate that the central supermassive black hole may have formed before its host galaxy. -
Catherino, William (Ed.)Objective: To assess whether co-culture with vitrified-warmed cumulus cells (CCs) in media drops improves rescue in vitro maturation (IVM) of previously vitrified immature oocytes. Previous studies have shown improved rescue IVM of fresh immature oocytes when cocultured with CCs in a three-dimensional matrix. However, the scheduling and workload of embryologists would benefit from a simpler IVM approach, particularly in the setting of time-sensitive oncofertility oocyte cryopreservation (OC) cases. Although the yield of developmentally competent mature metaphase II (MII) oocytes is increased when rescue IVM is performed before cryopreservation, it is unknown whether maturation of previously vitrified immature oocytes is improved after coculture with CCs in a simple system not involving a three-dimensional matrix. Design: Randomized controlled trial. Setting: Academic hospital. Patients: A total of 320 (160 germinal vesicles [GVs] and 160 metaphase I [MI]) immature oocytes and autologous CC clumps were vitrified from patients who were undergoing planned OC or intracytoplasmic sperm injection from July 2020 until September 2021. Interventions: On warming, the oocytes were randomized to culture in IVM media with CCs (+CC) or without CCs (-CC). Germinal vesicles and MI oocytes were cultured in 25 μL (SAGE IVM medium) for 32 hours and 20-22 hours, respectively. Main outcome measures: Oocytes with a polar body (MII) were randomized to confocal microscopy for analysis of spindle integrity and chromosomal alignment to assess nuclear maturity or to parthenogenetic activation to assess cytoplasmic maturity. Wilcoxon rank sum tests for continuous variables and the chi square or Fisher's exact test for categorical variables assessed statistical significance. Relative risks (RRs) and 95% confidence intervals (CIs) were calculated. Results: Patient demographic characteristics were similar for both the GV and MI groups after randomization to +CC vs. -CC. No statistically significant differences were observed between +CC vs. -CC groups regarding the percentage of MII from either GV (42.5% [34/80] vs. 52.5% [42/80]; RR 0.81; 95% CI: 0.57-1.15]) or MI (76.3% [61/80]; vs. 72.5% [58/80]; RR 1.05; 95% CI: 0.88-1.26]) oocytes. An increased percentage of GV-matured MIIs underwent parthenogenetic activation in the +CC group (92.3% [12/13] vs. 70.8% [17/24]), but the difference was not statistically significant (RR 1.30; 95% CI: 0.97-1.75), whereas the activation rate was identical for MI-matured oocytes (74.3% [26/35] vs. 75.0% [18/24], CC+ vs. CC-; RR 0.99; 95% CI: 0.74-1.32). No significant differences were observed between +CC vs. -CC groups for cleavage of parthenotes from GV-matured oocytes (91.7% [11/12] vs. 82.4% [14/17]) or blastulation (0 for both) or for MI-matured oocytes (cleavage: 80.8% [21/26] vs. 94.4% [17/18]; blastulation: 0 [0/26] vs. 16.7% [3/18]). Further, no significant differences were observed between +CC vs. -CC for GV-matured oocytes regarding incidence of bipolar spindles (38.9% [7/18] vs. 33.3% [5/15]) or aligned chromosomes (22.2% [4/18] vs. 0.0 [0/15]); or for MI-matured oocytes (bipolar spindle: 38.9% [7/18] vs. 42.9% [2/28]); aligned chromosomes (35.3% [6/17] vs. 24.1% [7/29]). Conclusions: Cumulus cell co-culture in this simple two-dimensional system does not improve rescue IVM of vitrified, warmed immature oocytes, at least by the markers assessed here. Further work is required to assess the efficacy of this system given its potential to provide flexibility in a busy, in vitro fertilization clinic.more » « less
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Abstract Background Meiosis is a specialized cell division that underpins sexual reproduction in most eukaryotes. During meiosis, interhomolog meiotic recombination facilitates accurate chromosome segregation and generates genetic diversity by shuffling parental alleles in the gametes. The frequency of meiotic recombination in
Arabidopsis has a U-shaped curve in response to environmental temperature, and is dependent on the Type I, crossover (CO) interference-sensitive pathway. The mechanisms that modulate recombination frequency in response to temperature are not yet known.Results In this study, we compare the transcriptomes of thermally-stressed meiotic-stage anthers from
msh4 andmus81 mutants that mediate the Type I and Type II meiotic recombination pathways, respectively. We show that heat stress reduces the number of expressed genes regardless of genotype. In addition,msh4 mutants have a distinct gene expression pattern compared tomus81 and wild type controls. Interestingly,ASY1, which encodes a HORMA domain protein that is a component of meiotic chromosome axes, is up-regulated in wild type andmus81 but not inmsh4 . In addition,SDS the meiosis-specific cyclin-like gene,DMC1 the meiosis-specific recombinase,SYN1/REC8 the meiosis-specific cohesion complex component, andSWI1 which functions in meiotic sister chromatid cohesion are up-regulated in all three genotypes. We also characterize 51 novel, previously unannotated transcripts, and show that their promoter regions are associated with A-rich meiotic recombination hotspot motifs.Conclusions Our transcriptomic analysis of
msh4 andmus81 mutants enhances our understanding of how the Type I and Type II meiotic CO pathway respond to environmental temperature stress and might provide a strategy to manipulate recombination levels in plants.
