Bioengineered in vitro models of the kidney offer unprecedented opportunities to better mimic the in vivo microenvironment. Kidney-on-a-chip technology reproduces 2D or 3D features which can replicate features of the tissue architecture, composition, and dynamic mechanical forces experienced by cells in vivo. Kidney cells are exposed to mechanical stimuli such as substrate stiffness, shear stress, compression, and stretch, which regulate multiple cellular functions. Incorporating mechanical stimuli in kidney-on-a-chip is critically important for recapitulating the physiological or pathological microenvironment. This review will explore approaches to applying mechanical stimuli to different cell types using kidney-on-a-chip models and how these systems are used to study kidney physiology, model disease, and screen for drug toxicity. We further discuss sensor integration into kidney-on-a-chip for monitoring cellular responses to mechanical or other pathological stimuli. We discuss the advantages, limitations, and challenges associated with incorporating mechanical stimuli in kidney-on-a-chip models for a variety of applications. Overall, this review aims to highlight the importance of mechanical stimuli and sensor integration in the design and implementation of kidney-on-a-chip devices.
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Strut‐Based Cellular to Shellular Funicular Materials
Owing to the fact that effective properties of low‐density cellular solids heavily rely on their underlying architecture, a variety of explicit and implicit techniques exists for designing cellular geometries. However, most of these techniques fail to present a correlation among architecture, internal forces, and effective properties. This paper introduces an alternative design strategy based on the static equilibrium of forces, equilibrium of polyhedral frames, and reciprocity of form and force. This novel approach reveals a geometric relationship among the truss system architecture, topological dual, and equilibrium of forces on the basis of 3D graphic statics. This technique is adapted to devise periodic strut‐based cellular architectures under certain boundary conditions and they are manipulated to construct shell‐based (shellular) cells with a variety of mechanical properties. By treating the materialized unit cells as representative volume elements (RVE), multiscale homogenization is used to investigate their effective linear elastic properties. Validated by experimental tests on 3D printed funicular materials, it is shown that by manipulating the RVE topology using the proposed methodology, alternative strut materialization schemes, and rational addition of bracing struts, cellular mechanical metamaterials can be systematically architected to demonstrate properties ranging from bending‐ to stretching‐dominated, realize metafluidic behavior, or create novel hybrid shellulars.
more » « less- NSF-PAR ID:
- 10446435
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Advanced Functional Materials
- Volume:
- 32
- Issue:
- 14
- ISSN:
- 1616-301X
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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