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Title: Recent advances (2019–2021) of capillary electrophoresis‐mass spectrometry for multilevel proteomics
Multilevel proteomics aims to delineate proteins at the peptide (bottom-up proteomics), proteoform (top-down proteomics), and protein complex (native proteomics) levels. Capillary electrophoresis-mass spectrometry (CE-MS) can achieve highly efficient separation and highly sensitive detection of complex mixtures of peptides, proteoforms, and even protein complexes because of its substantial technical progress. CE-MS has become a valuable alternative to the routinely used liquid chromatography-mass spectrometry for multilevel proteomics. This review summarizes the most recent (2019-2021) advances of CE-MS for multilevel proteomics regarding technological progress and biological applications. We also provide brief perspectives on CE-MS for multilevel proteomics at the end, highlighting some future directions and potential challenges.  more » « less
Award ID(s):
1846913
PAR ID:
10323792
Author(s) / Creator(s):
; ; ; ; ; ; ;
Date Published:
Journal Name:
Mass Spectrometry Reviews
ISSN:
0277-7037
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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