The constraints that govern the evolution of gene expression patterns across development remain unclear. Single-cell RNA sequencing can detail these constraints by systematically profiling homologous cells. The conserved invariant embryonic lineage ofCaenorhabditis elegansandC. briggsaemakes them ideal for comparing cell type gene expression across evolution. Measuring the spatiotemporal divergence of gene expression across embryogenesis, we find a high level of similarity in gene expression programs between species despite tens of millions of years of evolutionary divergence. Nonetheless, thousands of genes show divergence in their cell type specific expression patterns, with enrichment for functions in environmental response and behavior. Neuronal cell types show higher divergence than others such as the intestine and germline. This work identifies likely constraints on the evolution of developmental gene expression.
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Assembly and Evolution of Artificial Metalloenzymes within E. coli Nissle 1917 for Enantioselective and Site-Selective Functionalization of C─H and C═C Bonds
- Award ID(s):
- 2027943
- PAR ID:
- 10341333
- Date Published:
- Journal Name:
- Journal of the American Chemical Society
- Volume:
- 144
- Issue:
- 2
- ISSN:
- 0002-7863
- Page Range / eLocation ID:
- 883 to 890
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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