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Significant research on deep neural networks, culminating in AlphaFold2, convincingly shows that deep learning can predict the na- tive structure of a given protein sequence with high accuracy. In contrast, work on deep learning frameworks that can account for the structural plasticity of protein molecules remains in its infancy. Many researchers are now investigating deep generative models to explore the structure space of a protein. Current models largely use 2D convolution, leveraging representations of protein structures as contact maps or distance matri- ces. The goal is exclusively to generate protein-like, sequence-agnostic tertiary structures, but no rigorous metrics are utilized to convincingly make this case. This paper makes several contributions. It builds on momentum in graph representation learning and formalizes a protein tertiary structure as a contact graph. It demonstrates that graph repre- sentation learning outperforms models based on image convolution. This work also equips graph-based deep latent variable models with the abil- ity to learn from experimentally-available tertiary structures of proteins of varying lengths. The resulting models are shown to outperform state- of-the-art ones on rigorous metrics that quantify both local and distal patterns in physically-realistic protein structures. We hope this work will spur further research in deep generative models for obtaining a broader view of the structure space of a protein molecule.
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Data Size and Quality Matter: Generating Physically-Realistic Distance Maps of Protein Tertiary Structures
With the debut of AlphaFold2, we now can get a highly-accurate view of a reasonable equilibrium tertiary structure of a protein molecule. Yet, a single-structure view is insufficient and does not account for the high structural plasticity of protein molecules. Obtaining a multi-structure view of a protein molecule continues to be an outstanding challenge in computational structural biology. In tandem with methods formulated under the umbrella of stochastic optimization, we are now seeing rapid advances in the capabilities of methods based on deep learning. In recent work, we advance the capability of these models to learn from experimentally-available tertiary structures of protein molecules of varying lengths. In this work, we elucidate the important role of the composition of the training dataset on the neural network’s ability to learn key local and distal patterns in tertiary structures. To make such patterns visible to the network, we utilize a contact map-based representation of protein tertiary structure. We show interesting relationships between data size, quality, and composition on the ability of latent variable models to learn key patterns of tertiary structure. In addition, we present a disentangled latent variable model which improves upon the state-of-the-art variable autoencoder-based model in key, physically-realistic structural patterns. We believe this work opens up further avenues of research on deep learning-based models for computing multi-structure views of protein molecules.
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- PAR ID:
- 10342770
- Date Published:
- Journal Name:
- Biomolecules
- Volume:
- 12
- Issue:
- 7
- ISSN:
- 2218-273X
- Page Range / eLocation ID:
- 908
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.3390/biom12070908
