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Title: The Volumetric Properties of the Transition State Ensemble for Protein Folding
Hydrostatic pressure together with the temperature is an important environmental variable that plays an essential role in biological adaptation of extremophilic organisms. In particular, the effects of hy-drostatic pressure on the rates of the protein folding/unfolding reaction are determined by the magni-tude and sign of the activation volume changes. Here we provide computational description of the ac-tivation volume changes for folding/unfolding reaction, and compare them with the experimental data for six different globular proteins. We find that the volume of the transition state ensemble is always in-between the folded and unfolded states. Based on this, we conclude that hydrostatic pressure will invariably slow down protein folding and accelerate protein unfolding.  more » « less
Award ID(s):
1803045
NSF-PAR ID:
10342925
Author(s) / Creator(s):
Date Published:
Journal Name:
ChemRxiv
ISSN:
2573-2293
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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