Cdc48/p97 is an essential and highly conserved AAA+ ATPase that uses its protein-unfoldase activity to extract ubiquitinated polypeptides from macromolecular complexes and membranes. This motor has also been implicated in protein-degradation pathways, yet its exact role in acting upstream of the 26S proteasome remains elusive. Ubiquitinated proteins destined for degradation by the proteasome require an unstructured initiation region to engage with the proteasomal translocation machinery, and Cdc48 was proposed to generate these unfolded segments, yet direct evidence has been missing. Here, we used an in vitro reconstituted system to demonstrate the collaboration of Cdc48 and the 26S proteasome from
- Award ID(s):
- 1935596
- NSF-PAR ID:
- 10353425
- Date Published:
- Journal Name:
- Biomolecules
- Volume:
- 12
- Issue:
- 6
- ISSN:
- 2218-273X
- Page Range / eLocation ID:
- 750
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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