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Title: Single‐Lens Light‐Sheet Fluorescence Microscopy Based on Micro‐Mirror Array

Conventional light sheet fluorescence microscopy (LSFM) utilizes two perpendicularly arranged objective lenses for optical excitation and detection, respectively. Such a configuration often limits the use of high‐numerical‐aperture (NA) objectives or requires specially designed long‐working‐distance objectives. Here, a LSFM based on a micro‐mirror array (MMA) to enable light sheet imaging with a single objective lens is reported. The planar fluorescent emission excited by the light sheet illumination is collected by the same objective, relayed onto an MMA and detected by a side‐view camera. The proposed scheme makes LSFM compatible to single objective imaging system and shows promising candidacy for high spatiotemporal imaging.

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Author(s) / Creator(s):
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Publisher / Repository:
Wiley Blackwell (John Wiley & Sons)
Date Published:
Journal Name:
Laser & Photonics Reviews
Medium: X
Sponsoring Org:
National Science Foundation
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