The fundamental process of polarised exocytosis requires the interconnected activity of molecular motors trafficking vesicular cargo within a dynamic cytoskeletal network. In plants, few mechanistic details are known about how molecular motors, such as myosin XI, associate with their secretory cargo to support the ubiquitous processes of polarised growth and cell division. Live‐cell imaging coupled with targeted gene knockouts and a high‐throughput RNAi assay enabled the first characterisation of the loss of Rab‐E function. Yeast two‐hybrid and subsequent Rab‐E co‐localises with myosin XI at sites of active exocytosis, and at the growing tip both proteins are spatiotemporally coupled. Rab‐E is required for normal plant growth in Our results suggest that the interaction of Rab‐E and myosin XI is a conserved feature of polarised growth in plants.
Quantitative information on the spatiotemporal distribution of polarised proteins is central for understanding cell‐fate determination, yet collecting sufficient data for statistical analysis is difficult to accomplish with manual measurements. Here we present Polarity Measurement (P P Our results demonstrate that P
- Award ID(s):
- 1942722
- NSF-PAR ID:
- 10372496
- Publisher / Repository:
- Wiley-Blackwell
- Date Published:
- Journal Name:
- New Phytologist
- Volume:
- 230
- Issue:
- 2
- ISSN:
- 0028-646X
- Page Range / eLocation ID:
- p. 867-877
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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