More Like this

1. Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

   https://doi.org/10.3791/59914  

   Rosenberg, Jillian ; Huang, Jun ( January 2020 , Journal of Visualized Experiments)

   null (Ed.)
2. Lattice Light-Sheet Microscopy Multi-dimensional Analyses (LaMDA) of T-Cell Receptor Dynamics Predict T-Cell Signaling States

   https://doi.org/10.1016/j.cels.2020.04.006  

   Rosenberg, Jillian ; Cao, Guoshuai ; Borja-Prieto, Fernanda ; Huang, Jun ( May 2020 , Cell Systems)

   null (Ed.)
3. Vector field controlled vortex lattice symmetry in LiFeAs using scanning tunneling microscopy

   https://doi.org/10.1103/PhysRevB.99.161103  

   Zhang, Songtian S. ; Yin, Jia-Xin ; Dai, Guangyang ; Zheng, Hao ; Chang, Guoqing ; Belopolski, Ilya ; Wang, Xiancheng ; Lin, Hsin ; Wang, Ziqiang ; Jin, Changqing ; et al ( April 2019 , Physical Review B)
4. The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy

   https://doi.org/10.1038/s41467-021-25187-1  

   Quinn, Shayne E. ; Huang, Lu ; Kerkvliet, Jason G. ; Swanson, Joel A. ; Smith, Steve ; Hoppe, Adam D. ; Anderson, Robert B. ; Thiex, Natalie W. ; Scott, Brandon L. ( August 2021 , Nature Communications)

   Macropinosomes are formed by shaping actin-rich plasma membrane ruffles into large intracellular organelles in a phosphatidylinositol 3-kinase (PI3K)-coordinated manner. Here, we utilize lattice lightsheet microscopy and image visualization methods to map the three-dimensional structure and dynamics of macropinosome formation relative to PI3K activity. We show that multiple ruffling morphologies produce macropinosomes and that the majority form through collisions of adjacent PI3K-rich ruffles. By combining multiple volumetric representations of the plasma membrane structure and PI3K products, we show that PI3K activity begins early throughout the entire ruffle volume and continues to increase until peak activity concentrates at the base of the ruffle after the macropinosome closes. Additionally, areas of the plasma membrane rich in ruffling had increased PI3K activity and produced many macropinosomes of various sizes. Pharmacologic inhibition of PI3K activity had little effect on the rate and morphology of membrane ruffling, demonstrating that early production of 3′-phosphoinositides within ruffles plays a minor role in regulating their morphology. However, 3′-phosphoinositides are critical for the fusogenic activity that seals ruffles into macropinosomes. Taken together, these data indicate that local PI3K activity is amplified in ruffles and serves as a priming mechanism for closure and sealing of ruffles into macropinosomes.

    

   more » « less
5. Imaging Photon Lattice States by Scanning Defect Microscopy

   https://doi.org/10.1103/PhysRevX.6.021044  

   Underwood, D. L. ; Shanks, W. E. ; Li, Andy C. ; Ateshian, Lamia ; Koch, Jens ; Houck, A. A. ( June 2016 , Physical Review X)