skip to main content
US FlagAn official website of the United States government
dot gov icon
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
https lock icon
Secure .gov websites use HTTPS
A lock ( lock ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Explore Research Products in the PAR
It may take a few hours for recently added research products to appear in PAR search results.


Title: Microbial Indicators of Fecal Pollution: Recent Progress and Challenges in Assessing Water Quality
Abstract Purpose of Review Fecal contamination of water is a major public health concern. This review summarizes recent developments and advancements in water quality indicators of fecal contamination. Recent Findings This review highlights a number of trends. First, fecal indicators continue to be a valuable tool to assess water quality and have expanded to include indicators able to detect sources of fecal contamination in water. Second, molecular methods, particularly PCR-based methods, have advanced considerably in their selected targets and rigor, but have added complexity that may prohibit adoption for routine monitoring activities at this time. Third, risk modeling is beginning to better connect indicators and human health risks, with the accuracy of assessments currently tied to the timing and conditions where risk is measured. Summary Research has advanced although challenges remain for the effective use of both traditional and alternative fecal indicators for risk characterization, source attribution and apportionment, and impact evaluation.  more » « less
Award ID(s):
1316318
PAR ID:
10379792
Author(s) / Creator(s):
;
Date Published:
Journal Name:
Current Environmental Health Reports
Volume:
7
Issue:
3
ISSN:
2196-5412
Page Range / eLocation ID:
311 to 324
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. ; ; ; ; (, Sustainability)
    null (Ed.)
    Low-cost, field-deployable, near-time methods for assessing water quality are not available when and where waterborne infection risks are greatest. We describe the development and testing of a novel device for the measurement of tryptophan-like fluorescence (TLF), making use of recent advances in deep-ultraviolet light emitting diodes (UV-LEDs) and sensitive semiconductor photodiodes and photomultipliers. TLF is an emerging indicator of water quality that is associated with members of the coliform group of bacteria and therefore potential fecal contamination. Following the demonstration of close correlation between TLF and E. coli in model waters and proof of principle with sensitivity of 4 CFU/mL for E. coli, we further developed a two-LED flow-through configuration capable of detecting TLF levels corresponding to “high risk” fecal contamination levels (>10 CFU/100 mL). Findings to date suggest that this device represents a scalable solution for remote monitoring of drinking water supplies to identify high-risk drinking water in near-time. Such information can be immediately actionable to reduce risks. 
    more » « less
  2. ; ; ; ; ; ; ; ; ; (, Microbiology Spectrum)
    Gralnick, Jeffrey A. (Ed.)
    ABSTRACT The use of enterococci as a fecal indicator bacterial group for public health risk assessment has been brought into question by recent studies showing that “naturalized” populations of Enterococcus faecalis exist in the extraenteric environment. The extent to which these naturalized E. faecalis organisms can confound water quality monitoring is unclear. To determine if strains isolated from different habitats display different survival strategies and responses, we compared the decay patterns of three E. faecalis isolates from the natural environment (environmental strains) against three human gut isolates (enteric strains) in laboratory mesocosms that simulate an oligotrophic, aerobic freshwater environment. Our results showed similar overall decay rates between enteric and environmental isolates based on viable plate and quantitative PCR (qPCR) counts. However, the enteric isolates exhibited a spike in copy number ratios of 16S rRNA gene transcripts to 16S rRNA gene DNA copies (rRNA:rDNA ratios) between days 1 and 3 of the mesocosm incubations that was not observed in environmental isolates, which could indicate a different stress response. Nevertheless, there was no strong evidence of differential gene expression between environmental and enteric isolates related to habitat adaptation in the accompanying mesocosm metatranscriptomes. Overall, our results provide novel information on how rRNA levels may vary over different growth conditions (e.g., standard lab versus oligotrophic) for this important indicator bacteria. We also observed some evidence for habitat adaptation in E. faecalis ; however, this adaptation may not be substantial or consistent enough for integration in water quality monitoring. IMPORTANCE Enterococci are commonly used worldwide to monitor environmental fecal contamination and public health risk for waterborne diseases. However, closely related enterococci strains adapted to living in the extraenteric environment may represent a lower public health risk and confound water quality estimates. We developed an rRNA:rDNA viability assay for E. faecalis (a predominant species within this fecal group) and tested it against both enteric and environmental isolates in freshwater mesocosms to assess whether this approach can serve as a more sensitive water quality monitoring tool. We were unable to reliably distinguish the different isolate types using this assay under the conditions tested; thus, environmental strains should continue to be counted during routine water monitoring. However, this assay could be useful for distinguishing more recent (i.e., higher-risk) fecal pollution because rRNA levels significantly decreased after 1 week in all isolates. 
    more » « less
  3. ; ; (, Environmental Science: Water Research & Technology)
    Fecal indicator bacteria currently used for water quality monitoring inadequately represent viral fate in water systems, motivating the development of viral fecal pollution indicators. Molecular viral fecal pollution indicators such as crAssphage and pepper mild mottle virus (PMMoV) have emerged as leading viral fecal pollution indicator candidates due to ease and speed of measurement and target specificity. Elucidating the fate of molecular viral fecal indicators in water systems is necessary to facilitate their development, broader adoption, and ultimately their association with infectious risk. A significant mechanism controlling the behavior of viral indicators in environmental waters is association with particles, as this would dictate removal via settling and transport characteristics. In this study, we investigated the particle associations of six molecular fecal pollution targets (crAssphage, PMMoV, adenovirus, human polyomavirus, norovirus, HF183/BacR287) in wastewater using a cascade filtration approach. Four different filters were employed representing large settleable particles (180 μm), larger (20 μm) and smaller suspended particles (0.45 μm), and non-settleable particles (0.03 μm). All molecular targets were detected on all particle size fractions; however, all targets had their highest concentrations on the 0.45 μm (percent contribution ranging from 40% to 80.5%) and 20 μm (percent contribution ranging from 3.9% to 39.4%) filters. The association of viral fecal pollution targets with suspended particles suggests that particle association will dictate transport in environmental waters and that sample concentration approaches based upon particle collection will be effective for these targets. 
    more » « less
  4. ; (, Nitrogen)
    Nitrogen species present in the atmosphere, soil, and water play a vital role in ecosystem stability. Reactive nitrogen gases are key air quality indicators and are responsible for atmospheric ozone layer depletion. Soil nitrogen species are one of the primary macronutrients for plant growth. Species of nitrogen in water are essential indicators of water quality, and they play an important role in aquatic environment monitoring. Anthropogenic activities have highly impacted the natural balance of the nitrogen species. Therefore, it is critical to monitor nitrogen concentrations in different environments continuously. Various methods have been explored to measure the concentration of nitrogen species in the air, soil, and water. Here, we review the recent advancements in optical and electrochemical sensing methods for measuring nitrogen concentration in the air, soil, and water. We have discussed the advantages and disadvantages of the existing methods and the future prospects. This will serve as a reference for researchers working with environment pollution and precision agriculture. 
    more » « less
  5. ; ; (, The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021))
    A smartphone-integrated dielectrophoretic (SiDEP) platform is presented for on-site and real-time monitoring of fecal indicator bacteria (FIB) to examine the presence and concentration levels of fecal contamination in environmental water via loop-mediated isothermal amplification (LAMP) assays on a smartphone. Experimental demonstrations have verified the SiDEP’s capabilities for (1) on-chip water sample processing, (2) portable LAMP assays, and (3) colorimetric analysis of fecal water quality. The SiDEP truly offers a low-cost, portable, and fully-integrated system enabling rapid on-site detection of the presence of FIB and their associated pathogens in environmental water without the need for sophisticated laboratory equipment or skilled personnel. 
    more » « less
  • Citation Formats
  • MLA
  • APA
  • Chicago
  • BibTeX
  • Save / Share this Record
  • Send to Email