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Title: To twist or not to twist: From chromophore structure to dynamics inside engineered photoconvertible and photoswitchable fluorescent proteins
Award ID(s):
1817949 2003550
PAR ID:
10385977
Author(s) / Creator(s):
; ; ; ; ; ;
Date Published:
Journal Name:
Protein Science
Volume:
32
Issue:
1
ISSN:
0961-8368
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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  1. Abstract

    In less than a decade, CRISPR screening has revolutionized forward genetics and cell and molecular biology. Advances in screening technologies, including sgRNA libraries, Cas9‐expressing cell lines, and streamlined sequencing pipelines, have democratized pooled CRISPR screens at genome‐wide scale. Initially, many such screens were survival‐based, identifying essential genes in physiological or perturbed processes. With the application of new chemical biology tools to CRISPR screening, the phenotypic space is no longer limited to live/dead selection or screening for levels of conventional fluorescent protein reporters. Further, the resolution has been increased from cell populations to single cells or even the subcellular level. We highlight advances in pooled CRISPR screening, powered by chemical biology, that have expanded phenotypic space, resolution, scope, and scalability as well as strengthened the CRISPR/Cas enzyme toolkit to enable biological hypothesis generation and discovery.

     
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