genome size of 0.55 Mb, with an N 50 of 7,347 bp, estimated completeness of 88.16%, contamination and strain heterogeneity of 0%, and GC content of 25.1%. The Mollicutes MAG included 527 protein-coding genes and contained multiple rRNA genes (one each of 16S, 23S, and 5S). The MAG was most closely related to Mycoplasma marinum and Mycoplasma todarodis, which were isolated from an octopus and squid, respectively, based on a phylogenetic reconstruction from 63 CSCGs (Fig. 1; Table 1).

Data availability. The raw reads were deposited in the NCBI SRA database with accession numbers SRR12228976, SRR12228977, and SRR12228978, and the MAG assembly is available under ASM1966174v1, all attached to BioProject PRJNA646234.

Maximum-likelihood phylogeny based on conserved single-copy genes between the Mollicutes MAG from this study (PWAP), reference Mycoplasma genomes, and four genomes from the Firmicutes used as an outgroup. One hundred iterations were used to compute bootstrap values. The GenBank accession numbers for the genomes in the phylogenetic reconstruction are included in Table 1.