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We propose a novel cell segmentation approach by extracting Multi-exposure Maximally Stable Extremal Regions (MMSER) in phase contrast microscopy images on the same cell dish. Using our method, cell regions can be well identified by considering the maximally stable regions with response to different camera exposure times. Meanwhile, halo artifacts with regard to cells at different stages are leveraged to identify cells' stages. The experimental results validate that high quality cell segmentation and cell stage classification can be achieved by our approach.
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Synchronized strobed phase contrast and fluorescence microscopy: the interlaced standard reimagined
We propose a simple, cost-effective method for synchronized phase contrast and fluorescence video acquisition in live samples. Counter-phased pulses of phase contrast illumination and fluorescence excitation light are synchronized with the exposure of the two fields of an interlaced camera sensor. This results in a video sequence in which each frame contains both exposure modes, each in half of its pixels. The method allows real-time acquisition and display of synchronized and spatially aligned phase contrast and fluorescence image sequences that can be separated by de-interlacing in two independent videos. The method can be implemented on any fluorescence microscope with a camera port without needing to modify the optical path.
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- Award ID(s):
- 2146519
- PAR ID:
- 10394224
- Publisher / Repository:
- Optical Society of America
- Date Published:
- Journal Name:
- Optics Express
- Volume:
- 31
- Issue:
- 4
- ISSN:
- 1094-4087; OPEXFF
- Format(s):
- Medium: X Size: Article No. 5167
- Size(s):
- Article No. 5167
- Sponsoring Org:
- National Science Foundation
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