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Abstract The role of species interactions, as well as genetic and environmental factors, all likely contribute to the composition and structure of the gut microbiome; however, disentangling these independent factors under field conditions represents a challenge for a functional understanding of gut microbial ecology. Avian brood parasites provide unique opportunities to investigate these questions, as brood parasitism results in parasite and host nestlings being raised in the same nest, by the same parents. Here we utilized obligate brood parasite brown‐headed cowbird nestlings (BHCO;Molothrus ater) raised by several different host passerine species to better understand, via 16S rRNA sequencing, the microbial ecology of brood parasitism. First, we compared faecal microbial communities of prothonotary warbler nestlings (PROW;Protonotaria citrea) that were either parasitized or non‐parasitized by BHCO and communities among BHCO nestlings from PROW nests. We found that parasitism by BHCO significantly altered both the community membership and community structure of the PROW nestling microbiota, perhaps due to the stressful nest environment generated by brood parasitism. In a second dataset, we compared faecal microbiotas from BHCO nestlings raised by six different host passerine species. Here, we found that the microbiota of BHCO nestlings was significantly influenced by the parental host species and the presence of an inter‐specific nestmate. Thus, early rearing environment is important in determining the microbiota of brood parasite nestlings and their companion nestlings. Future work may aim to understand the functional effects of this microbiota variability on nestling performance and fitness.
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Do Attributes of Gray Vireo Nest Sites Influence Brown-headed Cowbird Brood Parasitism?
Brood parasitism can have major detrimental effects on the fitness of reproducing songbirds and often contributes to nest failure. As the Gray Vireo (Vireo vicinior) suffers high rates of brood parasitism by the Brown-headed Cowbird (Molothrus ater), we hypothesized that vireos might choose nest sites selectively to thwart cowbird parasitism. To investigate this question, we measured and compared vegetative and spatial attributes of nest sites in a known breeding population of the Gray Vireo on the Sevilleta National Wildlife Refuge, New Mexico, in 2017 and 2018. Specifically, we compared parasitized and unparasitized nests with respect to the nest’s height and the average height, diameter, and foliage density of the nest tree. We also compared the spatial attributes of the nearest neighboring nest, nearest parasitized nest, and the density of surrounding juniper trees within 50 m of a nest. Interestingly, among none of the variables measured did we find an association with the incidence of brood parasitism, suggesting that nest parasitism might be more strongly linked to other larger-scale ecological or behavioral variables.
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- Award ID(s):
- 1950237
- PAR ID:
- 10400013
- Date Published:
- Journal Name:
- Western Birds
- Volume:
- 53
- Issue:
- 1
- ISSN:
- 0045-3897
- Page Range / eLocation ID:
- 43 to 51
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.21199/WB53.1.4
