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When fluids flow through straight channels sustained turbulence occurs only at high Reynolds numbers [typically [Formula: see text]]. It is difficult to mix multiple fluids flowing through a straight channel in the low Reynolds number laminar regime [[Formula: see text]] because in the absence of turbulence, mixing between the component fluids occurs primarily via the slow molecular diffusion process. This Letter reports a simple way to significantly enhance the low Reynolds number (in our case [Formula: see text]) passive microfluidic flow mixing in a straight microchannel by introducing asymmetric wetting boundary conditions on the floor of the channel. We show experimentally and numerically that by creating carefully chosen two-dimensional hydrophobic slip patterns on the floor of the channels, we can introduce stretching, folding, and/or recirculation in the flowing fluid volume, the essential elements to achieve mixing in the absence of turbulence. We also show that there are two distinctive pathways to produce homogeneous mixing in microchannels induced by the inhomogeneity of the boundary conditions. It can be achieved either by (1) introducing stretching, folding and twisting of fluid volumes, i.e., via a horse-shoe type transformation map, or (2) by creating chaotic advection, achieved through manipulation of the hydrophobic boundary patterns on the floor of the channels. We have also shown that by superposing stretching and folding with chaotic advection, mixing can be optimized in terms of significantly reducing mixing length, thereby opening up new design opportunities for simple yet efficient passive microfluidic reactors.
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Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering
Advances in time-resolved structural techniques, mainly in macromolecular crystallography and small-angle X-ray scattering (SAXS), allow for a detailed view of the dynamics of biological macromolecules and reactions between binding partners. Of particular promise, are mix-and-inject techniques, which offer a wide range of experimental possibility as microfluidic mixers are used to rapidly combine two species just prior to data collection. Most mix-and-inject approaches rely on diffusive mixers, which have been effectively used within crystallography and SAXS for a variety of systems, but their success is dependent on a specific set of conditions to facilitate fast diffusion for mixing. The use of a new chaotic advection mixer designed for microfluidic applications helps to further broaden the types of systems compatible with time-resolved mixing experiments. The chaotic advection mixer can create ultra-thin, alternating layers of liquid, enabling faster diffusion so that even more slowly diffusing molecules, like proteins or nucleic acids, can achieve fast mixing on timescales relevant to biological reactions. This mixer was first used in UV–vis absorbance and SAXS experiments with systems of a variety of molecular weights, and thus diffusion speeds. Careful effort was also dedicated to making a loop-loading sample-delivery system that consumes as little sample as possible, enabling the study of precious, laboratory-purified samples. The combination of the versatile mixer with low sample consumption opens the door to many new applications for mix-and-inject studies.
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- Award ID(s):
- 1930046
- PAR ID:
- 10415117
- Date Published:
- Journal Name:
- IUCrJ
- Volume:
- 10
- Issue:
- 3
- ISSN:
- 2052-2525
- Page Range / eLocation ID:
- 363 to 375
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1107/S2052252523003482
