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Title: CCR4‐NOT subunit CCF ‐1/ CNOT7 promotes transcriptional activation to multiple stress responses in Caenorhabditis elegans

CCR4‐NOT is a versatile eukaryotic protein complex that controls multiple steps in gene expression regulation from synthesis to decay. In yeast, CCR4‐NOT has been implicated in stress response regulation, though this function in other organisms remains unclear. In a genome‐wide RNAi screen, we identified a subunit of the CCR4‐NOT complex,ccf‐1, as a requirement for theC. eleganstranscriptional response to cadmium and acrylamide stress. Using whole‐transcriptome RNA sequencing, we show that the knockdown ofccf‐1attenuates the activation of a broad range of stress‐protective genes in response to cadmium and acrylamide, including those encoding heat shock proteins and xenobiotic detoxification. Consistently, survival assays show that the knockdown ofccf‐1decreasesC. elegansstress resistance and normal lifespan. A yeast 2‐hybrid screen using a CCF‐1 bait identified the homeobox transcription factor PAL‐1 as a physical interactor. Knockdown ofpal‐1inhibits the activation ofccf‐1 dependent stress genes and reducesC. elegansstress resistance. Gene expression analysis reveals that knockdown ofccf‐1andpal‐1attenuates the activation ofelt‐2andelt‐3under stress that encode master transcriptional co‐regulators of stress response in theC. elegans, and that overexpression of ELT‐2 can suppressccf‐1's requirement for gene transcription in a stress‐dependent manner. Our findings reveal a new role for CCR4‐NOT in the environmental stress response and define its role in stress resistance and longevity inC. elegans.

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Journal Name:
Aging Cell
Medium: X
Sponsoring Org:
National Science Foundation
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    Graphical Abstract 
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  3. Summary

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  4. Abstract

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