Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) has become the gold‐standard technique to study RNA and its various modifications. While most research on RNA nucleosides has been focused on their biological roles, discovery of new modifications remains of interest. With state‐of‐the‐art technology, the presence of artifacts can confound the identification of new modifications. Here, we report the characterization of a non‐natural mcm5isoC ribonucleoside in
This content will become publicly available on May 10, 2024
- Award ID(s):
- 1904146
- NSF-PAR ID:
- 10428529
- Date Published:
- Journal Name:
- RSC Chemical Biology
- Volume:
- 4
- Issue:
- 5
- ISSN:
- 2633-0679
- Page Range / eLocation ID:
- 363 to 378
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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Abstract Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) has become the gold‐standard technique to study RNA and its various modifications. While most research on RNA nucleosides has been focused on their biological roles, discovery of new modifications remains of interest. With state‐of‐the‐art technology, the presence of artifacts can confound the identification of new modifications. Here, we report the characterization of a non‐natural mcm5isoC ribonucleoside in
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