Plant oils are valuable commodities for food, feed, renewable industrial feedstocks and biofuels. To increase vegetable oil production, here we show that the nonspecific phospholipase C6 (NPC6) promotes seed oil production in the Brassicaceae seed oil species Arabidopsis, Camelina and oilseed rape. Overexpression of Candidate‐gene association study in oilseed rape indicates that only These results indicate that NPC6 promotes membrane glycerolipid turnover to accumulate TAG production in oil seeds and that
Heteromeric acetyl‐CoA carboxylase (htACCase) catalyzes the committed step of
- NSF-PAR ID:
- 10446070
- Publisher / Repository:
- Wiley-Blackwell
- Date Published:
- Journal Name:
- The Plant Journal
- Volume:
- 110
- Issue:
- 4
- ISSN:
- 0960-7412
- Page Range / eLocation ID:
- p. 1035-1046
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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Summary NPC6 increased seed oil content, seed weight and oil yield both in Arabidopsis and Camelina, whereas knockout ofNPC6 decreased seed oil content and seed size. NPC6 is associated with the chloroplasts and microsomal membranes, and hydrolyzes phosphatidylcholine and galactolipids to produce diacylglycerol. Knockout and overexpression of NPC6 decreased and increased, respectively, the flux of fatty acids from phospholipids and galactolipids into triacylglycerol production.BnNPC6.C01 of the four homeologuesNPC6s is associated with seed oil content and yield. Haplotypic analysis indicates that theBnNPC6.C01 favorable haplotype can increase both seed oil content and seed yield.NPC6 has a great application potential for oil yield improvement. -
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