- PAR ID:
- 10448283
- Publisher / Repository:
- Nature Publishing Group
- Date Published:
- Journal Name:
- Nature Cell Biology
- Volume:
- 25
- Issue:
- 9
- ISSN:
- 1465-7392
- Page Range / eLocation ID:
- p. 1359-1368
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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Results NSCs cultured in three different concentrations of folate showed distinct mRNA methylation profiles. Despite uncovering only a few differentially expressed genes, hundreds of differentially translated genes were identified in NSCs with folate deficiency or supplementation. The differentially translated genes induced by low folate are associated with cytoplasmic translation and mitochondrial function, while the differentially translated genes induced by high folate are associated with increased neural stem cell proliferation. Interestingly, compared to total mRNAs, polysome mRNAs contained high levels of m5C. Furthermore, an integrative analysis indicated a transcript-specific relationship between RNA m5C methylation and mRNA translation efficiency.
Conclusions Altogether, our study reports a transcriptome-wide influence of folate on mRNA m5C methylation and translation in NSCs and reveals a potential link between mRNA m5C methylation and mRNA translation.
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