AbstractIn stroke, the sudden deprivation of oxygen to neurons triggers a profuse release of glutamate that induces anoxic depolarization (AD) and leads to rapid cell death. Importantly, the latency of the glutamate‐driven AD event largely dictates subsequent tissue damage. Although the contribution of synaptic glutamate during ischaemia is well‐studied, the role of tonic (ambient) glutamate has received far less scrutiny. The majority of tonic, non‐synaptic glutamate in the brain is governed by the cystine/glutamate antiporter, system xc−. Employing hippocampal slice electrophysiology, we showed that transgenic mice lacking a functional system xc−display longer latencies to AD and altered depolarizing waves compared to wild‐type mice after total oxygen deprivation. Experiments which pharmacologically inhibited system xc−, as well as those manipulating tonic glutamate levels and those antagonizing glutamate receptors, revealed that the antiporter's putative effect on ambient glutamate precipitates the ischaemic cascade. As such, the current study yields novel insight into the pathogenesis of acute stroke and may direct future therapeutic interventions.image Key pointsIschaemic stroke remains the leading cause of adult disability in the world, but efforts to reduce stroke severity have been plagued by failed translational attempts to mitigate glutamate excitotoxicity.Elucidating the ischaemic cascade, which within minutes leads to irreversible tissue damage induced by anoxic depolarization, must be a principal focus.Data presented here show that tonic, extrasynaptic glutamate supplied by system xc−synergizes with ischaemia‐induced synaptic glutamate release to propagate AD and exacerbate depolarizing waves.Exploiting the role of system xc−and its obligate release of ambient glutamate could, therefore, be a novel therapeutic direction to attenuate the deleterious effects of acute stroke.
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Comparative assessment of pre‐ and inter‐stage hydrothermal treatment of municipal sludge for increased methane production
Abstract Hydrothermal treatment (HT) is a promising technology to enhance anaerobic digestion (AD) of municipal sludge. However, the capacity of pre‐ and inter‐stage HT (i.e., HT‐AD and AD‐HT‐AD, respectively) to enhance the digestibility of municipal sludge has not been sufficiently explored. This study compared the efficacy of pre‐ and inter‐stage HT performed from 90 to 185°C to enhance methane production from a mixture of primary sludge and waste activated sludge using mesophilic (35°C) biochemical methane potential tests. In both configurations, sludge solubilization increased with HT temperature. HT‐AD, and to a greater extent AD‐HT‐AD, increased the release of ammonium nitrogen. Even though HT at 185°C dramatically increased sludge solubilization, the overall specific methane yield with HT at 185°C was lower than or comparable to that at lower HT temperatures in the HT‐AD and AD‐HT‐AD configurations, respectively. Up to 155°C HT, the overall specific methane yield with the HT‐AD configuration was higher by 4.9%–8.3% compared to the AD‐HT‐AD configuration. However, when the HT energy was considered, compared to the control (i.e., AD of sludge without HT), the net energy gain (ΔE) decreased as the HT temperature increased, becoming negative at an HT of 185°C. The AD‐HT‐AD configuration resulted in a higher overall volatile solids destruction (by 8.1 to 20.1%). In conclusion, for municipal sludge with a relatively high ultimate digestibility, as was the case in this study, HT‐AD is preferable as it has a smaller footprint and is easier to operate than the AD‐HT‐AD configuration. However, given the significantly higher volatile solids destruction in the AD‐HT‐AD configuration, compared to the HT‐AD configuration, AD‐HT‐AD may be more beneficial considering post‐AD sludge handling processes. Practitioner pointsHydrothermal treatment (HT) increased the rate and extent of methane production from municipal sludge mixture.155°C was the optimal temperature for either pre‐ or inter‐stage HT to increase biogas production.Pre‐ and inter‐stage HT resulted in comparable ultimate methane production.Pre‐stage HT is preferable to inter‐stage HT (smaller footprint, easier to operate).AD‐HT‐AD resulted in significantly higher volatile solids destruction compared to the HT‐AD configuration.
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- Award ID(s):
- 1739884
- PAR ID:
- 10449317
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Water Environment Research
- Volume:
- 93
- Issue:
- 7
- ISSN:
- 1061-4303
- Page Range / eLocation ID:
- p. 1126-1137
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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