The genus
Twenty‐four novel microsatellite markers (16 nuclear and eight chloroplast) were developed from
These markers are promising tools to study the population genetics of sexual
Eleven of eighteen Society Island
The genus
Twenty‐four novel microsatellite markers (16 nuclear and eight chloroplast) were developed from
These markers are promising tools to study the population genetics of sexual
Little is known about how diversification occurs within long‐lived, highly dispersible and continuously distributed groups. We examined the distribution of genetic variation within the woody genus
Hawaiian Islands.
We performed population genetic analyses of variation at nine nuclear microsatellite loci from 1,486 adults of 23
American Samoa and Tahiti populations clustered most closely with the older islands. Results also revealed isolation by distance across the archipelago, clustering of populations predominantly by island, and evidence of multiple colonizations or back‐colonizations of three islands. The number of genetic clusters peaked on islands of intermediate age, coincident with peak morphotype richness. All islands comprised a broad range of genetic distances among taxa with the greatest overall genetic distance observed on Oahu. The two taxa that are distributed broadly across the archipelago were weakly but significantly differentiated only on volcanically active Hawaii Island, where they partition early‐ and late‐successional environments. One of these taxa was positioned centrally both within individual‐island splitstree networks and across the archipelago‐wide network.
Distance‐dependent gene flow contributes to isolation of
Plant nuclear genomes harbor sequence elements derived from the organelles (mitochondrion and plastid) through intracellular gene transfer (IGT). Nuclear genomes also show a dramatic range of repeat content, suggesting that any sequence can be readily amplified. These two aspects of plant nuclear genomes are well recognized but have rarely been linked. Through investigation of 31
Genomic‐scale datasets, sophisticated analytical techniques, and conceptual advances have disproportionately failed to resolve species boundaries in some groups relative to others. To understand the processes that underlie taxonomic intractability, we dissect the speciation history of an Australian lizard clade that arguably represents a “worst‐case” scenario for species delimitation within vertebrates: the
Long‐tailed macaques (
We performed population genetic analyses and phylogenetic reconstruction on nuclear single nucleotide polymorphisms (SNPs) from shotgun sequencing of 75 long‐tailed macaque museum specimens from localities throughout Southeast Asia.
We show that shotgun sequencing of museum specimens yields sufficient genome coverage (average ~1.7%) for reconstructing population relationships using SNP data. Contrary to expectations of divergent results between nuclear and mitochondrial genomes for a female philopatric species, phylogeographical patterns based on nuclear SNPs proved to be closely similar to those found using mitogenomes. In particular, population genetic analyses and phylogenetic reconstruction from the nDNA identify two major clades within
Overall, we demonstrate that low‐coverage sequencing of nDNA from museum specimens provides enough data for examining broad phylogeographic patterns, although greater genome coverage and sequencing depth would be needed to distinguish between very closely related populations, such as those throughout the Philippines.