Ensuring undergraduate students become proficient in relating protein structure to biological function has important implications. With current two‐dimensional (2D) methods of teaching, students frequently develop misconceptions, including that proteins contain a lot of empty space, that bond angles for different amino acids can rotate equally, and that product inhibition is equivalent to allostery. To help students translate 2D images to 3D molecules and assign biochemical meaning to physical structures, we designed three 3D learning modules consisting of interactive activities with 3D printed models for amino acids, proteins, and allosteric regulation with coordinating pre‐ and post‐assessments. Module implementation resulted in normalized learning gains on module‐based assessments of 30% compared to 17% in a no‐module course and normalized learning gains on a comprehensive assessment of 19% compared to 3% in a no‐module course. This suggests that interacting with these modules helps students develop an improved ability to visualize and retain molecular structure and function.
Understanding the relationship between molecular structure and function represents an important goal of undergraduate life sciences. Although evidence suggests that handling physical models supports gains in student understanding of structure–function relationships, such models have not been widely implemented in biochemistry classrooms. Three‐dimensional (3D) printing represents an emerging cost‐effective means of producing molecular models to help students investigate structure–function concepts. We developed three interactive learning modules with dynamic 3D printed models to help biochemistry students visualize biomolecular structures and address particular misconceptions. These modules targeted specific learning objectives related to DNA and RNA structure, transcription factor‐DNA interactions, and DNA supercoiling dynamics. We also designed accompanying assessments to gauge student learning. Students responded favorably to the modules and showed normalized learning gains of 49% with respect to their ability to understand and relate molecular structures to biochemical functions. By incorporating accurate 3D printed structures, these modules represent a novel advance in instructional design for biomolecular visualization. We provide instructors with the materials necessary to incorporate each module in the classroom, including instructions for acquiring and distributing the models, activities, and assessments. © 2019 International Union of Biochemistry and Molecular Biology, 47(3):303–317, 2019.
more » « less- NSF-PAR ID:
- 10461520
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Biochemistry and Molecular Biology Education
- Volume:
- 47
- Issue:
- 3
- ISSN:
- 1470-8175
- Page Range / eLocation ID:
- p. 303-317
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
more » « less
Abstract -
more » « less
Abstract Biology and biochemistry students must learn to visualize and comprehend the complex three‐dimensional (3D) structures of macromolecules such as proteins or DNA. However, most tools available for teaching biomolecular structures typically operate in two dimensions. Here, we present protocols and pedagogical approaches for using immersive augmented reality (AR) visors, specifically the Microsoft HoloLens, to reinforce learning with large scale 3D holographic structures. We developed a novel workflow to render vividly colored custom biomolecules in AR visors. In addition, we developed AR exercises to review concepts relevant to protein or DNA structure and then implemented the exercises in four different biology and biochemistry courses. Surveys showed that students reported greater interest in biomolecular structures after the exercise. We also highlight some of the advantages and disadvantages of the software and hardware of this upcoming technology.
-
more » « less
Abstract Many STEM disciplines are underrepresented to High School students. This is problematic as many students' decisions for college are shaped by their experiences and achievements in high school. Short content‐oriented modules have been shown to encourage science identity and otherwise benefit the students' learning. Following the ASBMB's outreach protocol, we developed a short content‐oriented module aimed at a high school biology classroom. Students interacted with 3D models of DNA and transcription factors while exploring structure–function relationships and introductory biochemistry topics. The high school teacher was impressed with the students' response to the module, specifically the ease with which students learned, their enthusiasm, and their recall of the experience. We provide all materials necessary to use this module, including student worksheet and printable model coordinates. We encourage both high school instructors and professional biochemists to consider similar module using physical models.
-
Course-based Undergraduate Research Experiences (CUREs) are an increasingly utilized model for exposing students to research. The lack of robust assessments is a major hurdle to wider adoption of CUREs. The Coronavirus Infectious Disease 2019 (COVID-19) pandemic necessitated a drastic shift of in-person courses to the online format. Using the Participant Perception Indicator (PPI) survey, we measured students’ self-reported changes in learning from such a biochemistry course at a large university in south Florida based on the Biochemistry Authentic Scientific Inquiry Lab (BASIL) model. By doing this, we were able to better understand the student-benefits of CUREs and how these benefits are affected by changes in learning modalities between two relevant semesters, i.e., winter and summer of 2020. Anticipated learning outcomes (ALOs) help partially fill the gap left by the loss of physical interaction in experimental procedures. Our analysis indicated that students learned more through bioinformatic experiments compared to their wet-lab counterparts. Using pre- and post- surveys, students reported that their experience and confidence gains lagged behind their knowledge gain of technique-based skills. Students are not as confident in their understanding of techniques when unable to perform those in the physical laboratory. Thus, despite extensive pursuit of the purpose and protocols of the experiments and techniques, neither their experience nor their confidence was on par with their knowledge. This study is one of the first examples demonstrating a quantitative student-learning assessment of a CURE in the science, technology, engineering, and mathematics (STEM) disciplines. The novel assessment strategies targeted to identify gaps in learning mastery could facilitate the adoption of CUREs, fostering opportunities for all undergraduate students to vital laboratory research experiences in STEM.more » « less
-
null (Ed.)The development of tools that promote active learning in engineering disciplines is critical. It is widely understood that students engaged in active learning environments outperform those taught using passive methods. Previously, we reported on the development and implementation of hands-on Low-Cost Desktop Learning Modules (LCDLMs) that replicate real-world industrial equipment which serves to create active learning environments. Thus far, miniaturized venturi meter, hydraulic loss, and double-pipe and shell & tube heat exchanger DLMs have been utilized by hundreds of students across the country. It was demonstrated that the use of DLMs in face-to-face classrooms results in statistically significant improvements in student performance as well as increases in student motivation compared to students taught in a traditional lecture-only style classroom. Last year, participants in the project conducted 45 implementations including over 600 DLMs at 24 universities across the country reaching more than 1,000 students. In this project, we report on the significant progress made in broad dissemination of DLMs and accompanying pedagogy. We demonstrate that DLMs serve to increase student learning gains not only in face-to-face environments but also in virtual learning environments. Instructional videos were developed to aid in DLM-based learning during the COVID-19 pandemic when instructors were limited to virtual instruction. Preliminary results from this work show that students working with DLMs even in a virtual setting significantly outperform those taught without DLM-associated materials. Significant progress has also been made on the development of a new DLM cartridge: a see-through 3D-printed miniature fluidized bed. The new 3D printing methodology will allow for rapid prototyping and streamlined development of DLMs. A 3D-printed evaporative cooling tower DLM will also be developed in the coming year. In October 2020, the team held a virtual implementers workshop to train new participating faculty in DLM use and implementation. In total, 13 new faculty participants from 10 universities attended the 6-hour, 2-day workshop and plan to implement DLMs in their classrooms during this academic year. In the last year, this project was disseminated in 8 presentations at the American Society for Engineering Education (ASEE) Virtual Conference (June 2020) and American Institute of Chemical Engineers Annual Conference (November 2019) as well as the AIChE virtual Community of Practice Labs Group and a seminar at a major university, ultimately disseminating DLM pedagogy to approximately 200 individuals including approximately 120 university faculty. Further, the former group postdoc has accepted an instructor faculty position at University of Wisconsin Madison where she will teach unit operations among other subjects; she and the remainder of the team believe the LCDLM project has prepared her well for that position. In the remaining 2.5 years of the project, we will continue to evaluate the effectiveness of DLMs in teaching key heat transfer and fluid dynamics concepts thru implementations in the rapidly expanding pool of participating universities. Further, we continue our ongoing efforts in creating the robust support structure necessary for large-scale adoption of hands-on educational tools for promotion of hands-on interactive student learning.more » « less
