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Title: A computer vision based optical method for measuring fluid level in cell culture plates
For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid’s index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applied in the design of a 3D printable sensing device using a raspberry pi zero and a camera.  more » « less
Award ID(s):
2134955 2034037
PAR ID:
10464178
Author(s) / Creator(s):
;
Editor(s):
Hilgen, Gerrit
Publisher / Repository:
PLOS ONE
Date Published:
Journal Name:
PLOS ONE
Volume:
18
Issue:
9
ISSN:
1932-6203
Page Range / eLocation ID:
e0290951
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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