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Title: Robust and tunable performance of a cell-free biosensor encapsulated in lipid vesicles
Cell-free systems have enabled the development of genetically encoded biosensors to detect a range of environmental and biological targets. Encapsulation of these systems in synthetic membranes to form artificial cells can reintroduce features of the cellular membrane, including molecular containment and selective permeability, to modulate cell-free sensing capabilities. Here, we demonstrate robust and tunable performance of a transcriptionally regulated, cell-free riboswitch encapsulated in lipid membranes, allowing the detection of fluoride, an environmentally important molecule. Sensor response can be tuned by varying membrane composition, and encapsulation protects from sensor degradation, facilitating detection in real-world samples. These sensors can detect fluoride using two types of genetically encoded outputs, enabling detection of fluoride at the Environmental Protection Agency maximum contaminant level of 0.2 millimolars. This work demonstrates the capacity of bilayer membranes to confer tunable permeability to encapsulated, genetically encoded sensors and establishes the feasibility of artificial cell platforms to detect environmentally relevant small molecules.  more » « less
Award ID(s):
1844336 2145050 1844219
PAR ID:
10472759
Author(s) / Creator(s):
; ; ;
Date Published:
Journal Name:
Science Advances
Volume:
9
Issue:
1
ISSN:
2375-2548
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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