skip to main content
US FlagAn official website of the United States government
dot gov icon
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
https lock icon
Secure .gov websites use HTTPS
A lock ( lock ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.


Title: Microwave emissions from the cold atmospheric helium plasma jet
Abstract One of the recently observed effects of plasma in medical applications is the physical effect, suggesting that the electromagnetic (EM) emission of cold atmospheric plasmas can lead to cell membrane oscillations and sensitization to the chemical active ingredient of treatments such as cancer drugs. This is a new aspect that must be considered along with the plasma chemical effects for the future dose definition which is the most urgent research topic of plasma medicine. However, unlike the reactive oxygen and nitrogen species generated from plasma chemistry which is well-known as playing a key role in apoptosis cancer cells, the EM emission power spectrum and emission mechanism are still unquantified. This makes the uncertainty of the physical dosage of the therapy and thus impedes the further understanding and optimization of the plasma therapy. In this paper, we compute the 3D spatial distribution of the power density spectrum of EM emission from a cold atmospheric helium plasma jet. The simulations indicate that the plasma oscillations following the plasma streamer propagation are the main source of EM emission, while the emissions of the bulk current caused by net charge movements and the bremsstrahlung due to charge collisions are negligible. The results are also verified by a microwave power measurement using a heterodyne frequency sweep. These findings will thus fill out the last missing piece of the jigsaw before the plasma medicine community can define the dose in the future.  more » « less
Award ID(s):
1747760
PAR ID:
10489278
Author(s) / Creator(s):
; ;
Publisher / Repository:
IOP
Date Published:
Journal Name:
Plasma Sources Science and Technology
Volume:
32
Issue:
9
ISSN:
0963-0252
Page Range / eLocation ID:
095012
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. null (Ed.)
    Cold atmospheric plasma (CAP) is an ionized gas, the product of a non-equilibrium discharge at atmospheric conditions. Both chemical and physical factors in CAP have been demonstrated to have unique biological impacts in cancer treatment. From a chemical-based perspective, the anti-cancer efficacy is determined by the cellular sensitivity to reactive species. CAP may also be used as a powerful anti-cancer modality based on its physical factors, mainly EM emission. Here, we delve into three CAP cancer treatment approaches, chemically based direct/indirect treatment and physical-based treatment by discussing their basic principles, features, advantages, and drawbacks. This review does not focus on the molecular mechanisms, which have been widely introduced in previous reviews. Based on these approaches and novel adaptive plasma concepts, we discuss the potential clinical application of CAP cancer treatment using a critical evaluation and forward-looking perspectives. 
    more » « less
  2. null (Ed.)
    Cold atmospheric plasma (CAP), an ionized gas with near room temperature, shows a wide application in medicine. CAP is a tunable source of complex chemical components including many reactive species, which allows CAP to exert many biological effects on bacterial, fungal, yeast, and mammalian cells particularly cancer cells. In this review, we discuss the novel state of the art CAP-based cancer treatment. We focus on the comparison between the direct CAP treatment and the indirect CAP treatment which implements the use of CAP-activated solutions. The difference between the two treatment strategies reveals two unique features of the biological response to CAP: the cell-based H 2 O 2 generation and the activation phenomenon. Short-lived reactive species and physical factors from plasma may trigger these two cellular responses. 
    more » « less
  3. Abstract Cold atmospheric plasma (CAP), a near room temperature ionized gas, has shown potential application in many branches of medicine, particularly in cancer treatment. In previous studies, the biological effect of CAP on cancer cells and other mammalian cells has been based solely on the chemical factors in CAP, particularly the reactive species. Therefore, plasma medicine has been regarded as a reactive species-based medicine, and the physical factors in CAP such as the thermal effect, ultraviolet irradiation, and electromagnetic effect have been regarded as ignorable factors. In this study, we investigated the effect of a physical CAP treatment on glioblastoma cells. For the first time, we demonstrated that the physical factors in CAP could reinstate the positive selectivity on CAP-treated astrocytes. The positive selectivity was a result of necrosis, a new cell death in glioblastoma cells characterized by the leak of bulk water from the cell membrane. The physically-based CAP treatment overcomed a large limitation of the traditional chemically based CAP treatment, which had complete dependence on the sensitivity of cells to reactive species. The physically-based CAP treatment is a potential non-invasive anti-tumor tool, which may have wide application for tumors located in deeper tissues. 
    more » « less
  4. Plasma‐based biomedical applications rely on the reactive oxygen and nitrogen species generated in cold atmospheric plasmas, where complex chemical kinetic schemes occur. The optimization of plasma medicine is thus required for each specific biomedical purpose. In the view of pharmacology, it is to optimize the active pharmaceutical ingredients. This work is thus the first attempt of such a complex task utilizing the recent development of machine learning technologies. Herein, a general method of passive plasma chemical diagnostics and optimization in real time is proposed. Based on spontaneous emission spectroscopy, an artificial neural network provides the gas chemical compositions along with other information such as temperatures. The information further passes through the second neural network which outputs the adjustments of external control inputs including energy, gas injections, and extractions to optimize the plasma chemistry. 
    more » « less
  5. Abstract Cold atmospheric plasma (CAP) technology, a relatively novel technique mainly investigated as a stand-alone cancer treatment method in vivo and in vitro, is being proposed for application in conjunction with chemotherapy. In this study, we explore whether CAP, an ionized gas produced in laboratory settings and that operates at near room temperature, can enhance Temozolomide (TMZ) cytotoxicity on a glioblastoma cell line (U87MG). Temozolomide is the first line of treatment for glioblastoma, one of the most aggressive brain tumors that remains incurable despite advancements with treatment modalities. The cellular response to a single CAP treatment followed by three treatments with TMZ was monitored with a cell viability assay. According to the cell viability results, CAP treatment successfully augmented the effect of a cytotoxic TMZ dose (50 μM) and further restored the effect of a non-cytotoxic TMZ dose (10 μM). Application of CAP in conjunction TMZ increased DNA damage measured by the phosphorylation of H2AX and induced G2/M cell cycle arrest. These findings were supported by additional data indicating reduced cell migration and increased αvβ3 and αvβ5 cell surface integrin expression as a result of combined CAP–TMZ treatment. The data presented in this study serve as evidence that CAP technology can be a suitable candidate for combination therapy with existing chemotherapeutic drugs. CAP can also be investigated in future studies for sensitizing glioblastoma cells to TMZ and other drugs available in the market. 
    more » « less