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Abstract Light serves as a pivotal environmental cue regulating various aspects of plant growth and development, including seed germination, seedling de-etiolation, and shade avoidance. Within this regulatory framework, the basic helix–loop–helix transcription factors known as phytochrome-interacting factors (PIFs) play an essential role in orchestrating responses to light stimuli. Phytochromes, acting as red/far-red light receptors, initiate a cascade of events leading to the degradation of PIFs (except PIF7), thereby triggering transcriptional reprogramming to facilitate photomorphogenesis. Recent research has unveiled multiple post-translational modifications that regulate the abundance and/or activity of PIFs, including phosphorylation, dephosphorylation, ubiquitination, deubiquitination, and SUMOylation. Moreover, intriguing findings indicate that PIFs can influence chromatin modifications. These include modulation of histone 3 lysine 9 acetylation (H3K9ac), as well as occupancy of histone variants such as H2A.Z (associated with gene repression) and H3.3 (associated with gene activation), thereby intricately regulating downstream gene expression in response to environmental cues. This review summarizes recent advances in understanding the role of PIFs in regulating various signaling pathways, with a major focus on photomorphogenesis.
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Phytochrome F mediates red light responsiveness additively with phytochromes B1 and B2 in tomato
Abstract Phytochromes are red light and far-red light sensitive, plant-specific light receptors that allow plants to orient themselves in space and time. Tomato (Solanum lycopersicum) contains a small family of five phytochrome genes, for which to date stable knockout mutants are only available for three of them. Using CRISPR technology, we created multiple alleles of SlPHYTOCHROME F (phyF) mutants to determine the function of this understudied phytochrome. We report that SlphyF acts as a red/far-red light reversible low fluence sensor, likely through the formation of heterodimers with SlphyB1 and SlphyB2. During photomorphogenesis, phyF functions additively with phyB1 and phyB2. Our data further suggest that phyB2 requires the presence of either phyB1 or phyF during seedling de-etiolation in red light, probably via heterodimerization, while phyB1 homodimers are required and sufficient to suppress hypocotyl elongation in red light. During the end-of-day far-red response, phyF works additively with phyB1 and phyB2. In addition, phyF plays a redundant role with phyB1 in photoperiod detection and acts additively with phyA in root patterning. Taken together, our results demonstrate various roles for SlphyF during seedling establishment, sometimes acting additively, other times acting redundantly with the other phytochromes in tomato.
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- Award ID(s):
- 2038142
- PAR ID:
- 10489431
- Publisher / Repository:
- Oxford University Press
- Date Published:
- Journal Name:
- Plant Physiology
- Volume:
- 191
- Issue:
- 4
- ISSN:
- 0032-0889
- Page Range / eLocation ID:
- 2353 to 2366
- Subject(s) / Keyword(s):
- phytochrome
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1093/plphys/kiad028
