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Summary The non-muscle actomyosin cytoskeleton generates contractile force through the dynamic rearrangement of its constituent parts. Actomyosin rings are a specialization of the non-muscle actomyosin cytoskeleton that drive cell shape changes during division, wound healing, and other events. Contractile rings throughout phylogeny and in a range of cellular contexts are built from conserved components including non-muscle myosin II (NMMII), actin filaments (F-actin), and crosslinking proteins. However, it is unknown whether diverse actomyosin rings close via a single unifying mechanism. To explore how contractile forces are generated by actomyosin rings, we studied three instances of ring closure within the common cytoplasm of theC. elegansoogenic germline: mitotic cytokinesis of germline stem cells (GSCs), apoptosis of meiotic compartments, and cellularization of oocytes. We found that each ring type closed with unique kinetics, protein density and abundance dynamics. These measurements suggested that the mechanism of contractile force generation varied across the subcellular contexts. Next, we formulated a physical model that related the forces generated by filament-filament interactions to the material properties of these rings that dictate the kinetics of their closure. Using this framework, we related the density of conserved cytoskeletal proteins anillin and NMMII to the kinematics of ring closure. We fitted model rings to in situ measurements to estimate parameters that are currently experimentally inaccessible, such as the asymmetric distribution of protein along the length of F-actin, which occurs naturally due to differences in the dimensions of the crosslinker and NMMII filaments. Our work predicted that the role of NMMII varies across these ring types, due in part to its distribution along F-actin and motoring. Our model also predicted that the degree of contractility and the impact of ring material properties on contractility differs among ring types.
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Numerical Simulations of (10199) Chariklo’s Rings with a Resonant Perturber
Abstract The discovery of two thin rings around the ∼ 250 km sized Centaur Chariklo was the first of its kind, and their formation and evolutionary mechanisms are not well understood. Here, we explore a single shepherd satellite as a mechanism to confine Chariklo’s rings. We also investigate the impact of such a perturber on reaccretion, which is a likely process for material located outside the Roche limit. We have modifiedN-body code that was developed for Saturn’s rings to model the Chariklo system. Exploration of a reasonable parameter space indicates that rings like those observed could be stable as the result of a single satellite with a mass of a few ×1013kg that is in orbital resonance with the rings. There is a roughly linear relationship between the model optical depth and the mass of the satellite required to confine a ring. Ring particles do not accrete into moonlets during hard-sphere simulations. However, a reasonable fraction of the ring material forms into moonlets after a few tens of orbits for soft-sphere collisions. The ring-particle properties are thus key parameters in terms of moonlet accretion or destruction in this system.
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- Award ID(s):
- 2206306
- PAR ID:
- 10489704
- Publisher / Repository:
- DOI PREFIX: 10.3847
- Date Published:
- Journal Name:
- The Planetary Science Journal
- Volume:
- 5
- Issue:
- 2
- ISSN:
- 2632-3338
- Format(s):
- Medium: X Size: Article No. 32
- Size(s):
- Article No. 32
- Sponsoring Org:
- National Science Foundation
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