We introduce μVAST, a high-throughput acoustic microstreaming platform using second-order microstreaming to induce fluid transport and measure the viscosity of 16 samples, automating process flows in drug development, materials manufacturing and production.
more » « less- PAR ID:
- 10490315
- Editor(s):
- Wheeler, A.
- Publisher / Repository:
- Royal Society of Chemistry
- Date Published:
- Journal Name:
- Lab on a Chip
- Volume:
- 23
- Issue:
- 11
- ISSN:
- 1473-0197
- Page Range / eLocation ID:
- 2577 to 2585
- Subject(s) / Keyword(s):
- microfluidics
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
Microstreaming of acoustically excited bubbles presents great potential to mitigate fouling for membrane technologies. However, the acoustic streaming in bulk fluids under membrane separation conditions is not well explored. In this work, we investigate the microstreaming of 3D printed Helmholtz-like bubble-trapping structures (BTSs) under no flow, pressurized, and crossflow conditions that are relevant to membrane applications. Trapped bubbles are shown to generate formidable microstreaming that spans millimeter distances with velocity as high as 125 mm/s in a bulk aqueous medium. However, complex mode shapes of the bubble oscillation and bubble growth were observed during the frequency sweep. As a result, the streaming velocity decreases by 76% over 30 min, under single frequency excitation. The BTS displayed effective microstreaming under hydrostatic pressure up to 9.0 kPa, and under a crossflow velocity up to 0.2 mm/s, where the microstreaming zone reduced to <1 mm. The results provide the operation window, as well as challenges, for future integration of the BTS into bulk membrane separation processes.more » « less
-
The measurement of fluids viscosity represents a huge need for many biomedical and material applications. Sample fluids containing DNA, antibodies, proteins, and even cells have become important therapeutical options. The physical properties, including viscosity, of these biologics are critical factors in the optimization of the biomanufacturing processes. Here we demonstrate an acoustic microstreaming platform that induces fluid transport from second-order microstreaming. We validate our platform with water-glycerol mixture to reflect different viscosities and measure the maximum speed of the second-order acoustic microstreaming within seconds. Our demonstrated platform does not require external instruments to pump fluids and consumes small amount of fluid sample (< 10 uL) and is easy to incorporate with automation systems.more » « less
-
It has been previously reported that a gaseous bubble trapped in a one-end-open tube oscillates in the presence of acoustic wave and generates strong microstreaming flows and thus a propulsion force. The propulsion highly depends on the frequency and the voltage of the external acoustic wave. This paper presents a new discovery that the direction of this propulsion is dependent on the relative location of the bubble interface. The oscillating bubble propels forward when its interface stays deep inside the tube. On the contrary, the bubble propels in a reverse direction when its interface is at the exit of the tube. Learning from this phenomenon, we developed and introduced physical structures (necks) to precisely control the location of the bubble interface. As a result, the length and interface position of the bubble is more controllable, and the bubble oscillation and propulsion becomes more predictable and consistent.more » « less
-
Abstract Nanocarrier and exosome encapsulation has been found to significantly increase the efficacy of targeted drug delivery while also minimizing unwanted side effects. However, the development of exosome-encapsulated drug nanocarriers is limited by low drug loading efficiencies and/or complex, time-consuming drug loading processes. Herein, we have developed an acoustofluidic device that simultaneously performs both drug loading and exosome encapsulation. By synergistically leveraging the acoustic radiation force, acoustic microstreaming, and shear stresses in a rotating droplet, the concentration, and fusion of exosomes, drugs, and porous silica nanoparticles is achieved. The final product consists of drug-loaded silica nanocarriers that are encased within an exosomal membrane. The drug loading efficiency is significantly improved, with nearly 30% of the free drug (e.g., doxorubicin) molecules loaded into the nanocarriers. Furthermore, this acoustofluidic drug loading system circumvents the need for complex chemical modification, allowing drug loading and encapsulation to be completed within a matter of minutes. These exosome-encapsulated nanocarriers exhibit excellent efficiency in intracellular transport and are capable of significantly inhibiting tumor cell proliferation. By utilizing physical forces to rapidly generate hybrid nanocarriers, this acoustofluidic drug loading platform wields the potential to significantly impact innovation in both drug delivery research and applications.
-
Abstract A high‐throughput non‐viral intracellular delivery platform is introduced for the transfection of large cargos with dosage‐control. This platform, termed Acoustic‐Electric Shear Orbiting Poration (AESOP), optimizes the delivery of intended cargo sizes with poration of the cell membranes via mechanical shear followed by the modulated expansion of these nanopores via electric field. Furthermore, AESOP utilizes acoustic microstreaming vortices wherein up to millions of cells are trapped and mixed uniformly with exogenous cargos, enabling the delivery of cargos into cells with targeted dosages. Intracellular delivery of a wide range of molecule sizes (<1 kDa to 2 MDa) with high efficiency (>90%), cell viability (>80%), and uniform dosages (<60% coefficient of variation (CV)) simultaneously into 1 million cells min−1per single chip is demonstrated. AESOP is successfully applied to two gene editing applications that require the delivery of large plasmids: i) enhanced green fluorescent protein (eGFP) plasmid (6.1 kbp) transfection, and ii) clustered regularly interspaced short palindromic repeats (CRISPR)‐Cas9‐mediated gene knockout using a 9.3 kbp plasmid DNA encoding Cas9 protein and single guide RNA (sgRNA). Compared to alternative platforms, this platform offers dosage‐controlled intracellular delivery of large plasmids simultaneously to large populations of cells while maintaining cell viability at comparable delivery efficiencies.